Devlopment and validation of RP-HPLC Method for estimation of Metronidazole and Norfloxacin in suspension form
DOI:
https://doi.org/10.7439/ijap.v2i1.1403Keywords:
drug utilization, prescriptions, antimicrobials, rationalAbstract
A simple reversed- phase high-performance liquid chromatographic method (RP-HPLC) has been developed and validated for estimation of Development and Validation of Analytical method for Estimation of Metronidazole and Norfloxacin in pharmaceutical dosage form . Chromatographic separation was carried out on YL 9100 equipped with PDA detector using C 18 (250mm 4.6mm, 5) as stationary phase and mobile phase Triethylamine: 0.02M Potassium Dihydrogen phosphate (pH 3.5): Methanol (0.01:70:30) at flow rate of 1ml/min. Wavelength for UV detection was 292nm. The retention time for Metronidazole and Norfloxacin was found to be 6.10 and 3.50 min. The method was validated as per ICH guideline and and can be applied for estimation of Metronidazole and Norfloxacin in suspension. The linearity was found over concentration range of 12.5-37.5g/ml for Metronidazole and Norfloxacin.Downloads
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Published
2013-06-13
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Research Articles
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1.
Devlopment and validation of RP-HPLC Method for estimation of Metronidazole and Norfloxacin in suspension form. Int J Adv Pharm [Internet]. 2013 Jun. 13 [cited 2024 Sep. 8];2(1):05-11. Available from: https://ssjournals.co.in/index.php/ijap/article/view/1403