Devlopment and validation of RP-HPLC Method for estimation of Metronidazole and Norfloxacin in suspension form

Authors

  • Kuldip Dilipkumar Trivedi Saraswati Institute of Pharmaceutical Sciences, Dhanap, Gandhinagar, Gujarat
  • Ankit B Chaudhary Saraswati Institute of Pharmaceutical Sciences, Dhanap, Gandhinagar, Gujarat
  • Sellapan Mohan Saraswati Institute of Pharmaceutical Sciences, Dhanap, Gandhinagar, Gujarat

DOI:

https://doi.org/10.7439/ijap.v2i1.1403

Keywords:

drug utilization, prescriptions, antimicrobials, rational

Abstract

A simple reversed- phase high-performance liquid chromatographic method (RP-HPLC) has been developed and validated for estimation of Development and Validation of Analytical method for Estimation of Metronidazole and Norfloxacin in pharmaceutical dosage form . Chromatographic separation was carried out on YL 9100 equipped with PDA detector using C 18 (250mm 4.6mm, 5) as stationary phase and mobile phase Triethylamine: 0.02M Potassium Dihydrogen phosphate (pH 3.5): Methanol (0.01:70:30) at flow rate of 1ml/min. Wavelength for UV detection was 292nm. The retention time for Metronidazole and Norfloxacin was found to be 6.10 and 3.50 min. The method was validated as per ICH guideline and and can be applied for estimation of Metronidazole and Norfloxacin in suspension. The linearity was found over concentration range of 12.5-37.5g/ml for Metronidazole and Norfloxacin.

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Author Biography

  • Kuldip Dilipkumar Trivedi, Saraswati Institute of Pharmaceutical Sciences, Dhanap, Gandhinagar, Gujarat
    Quality Assurance

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Published

2013-06-13

Issue

Vol. 2 No. 1 (2013): Jan-Feb

Section

Research Articles

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How to Cite

1.
Devlopment and validation of RP-HPLC Method for estimation of Metronidazole and Norfloxacin in suspension form. Int J Adv Pharm [Internet]. 2013 Jun. 13 [cited 2026 Jan. 26];2(1):05-11. Available from: https://ssjournals.co.in/index.php/ijap/article/view/1403
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