Association of Glutathione S-transferase (GSTM1, GSTT1 and GSTP1) polymorphisms and passive smoking in cervical cancer cases from North India

Authors

  • Mohammad Abbas Molecular and Human Genetics Laboratory, Department of Zoology, University of Lucknow, Lucknow -226007, Uttar Pradesh
  • Kirti Srivastava Department of Radiotherapy, King George
  • Mohd. Imran Department of Microbiology, Integral University, Lucknow-226026, Uttar Pradesh
  • Monisha Banerjee Molecular and Human Genetics Laboratory, Department of Zoology, University of Lucknow, Lucknow.

DOI:

https://doi.org/10.7439/ijbr.v4i12.396

Keywords:

ABO Blood Group, Rh factor, blood donors

Abstract

Objectives: To evaluate association of genetic polymorphisms in GSTM1 , T1 , and P1 genes with cervical cancer (CC) susceptibility and effect of passive smoking. Methods: The polymorphisms in GSTM1 and T1 were analyzed by multiplex polymerase chain reaction (mPCR) and GSTP1 by PCR - Restriction Fragment Length Polymorphism (PCR-RFLP) in 150 CC cases and 165 healthy controls. Results: CC cases showed significant association with marriage age, parity and hemoglobin level (p 0.0001). GSTM1 (-/-) genotype was prominent among cases (30.0 %) whereas on combined analysis, GSTM1 (-/-) / T1 (-/-) and GSTM1 (-/-) / P1 (A/G or G/G) genotypes showed 2.75 and 2.98 times higher susceptibility to CC respectively. Triple combinations of GSTM1 (-/-), T1 (+/+) and P 1 (A/G or G/G) genotypes showed significant association ( P =0.02) with a 3.04 times risk of developing CC. Further analysis of cervical cancer histological subtypes such as squamous cell carcinoma and adenocarcinoma revealed an association of GSTM1 (-/-), GSTP1 A/G and G/G genotypes in adenocarcinoma cases alone (4.01, 2.25 and 2.40 folds respectively). Out of all the CC cases, passive smokers with GSTM1 (-/-) and GSTP1 (G/G) genotypes showed 4.19 and 2.88 times respectively of increased risk of developing the disease. Conclusions: This study confirms the possible interactions between different GST polymorphisms and passive smoking in cervical cancer cases from North India.

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Published

2013-12-30

Issue

Vol. 4 No. 12 (2013): Dec

Section

Original Research Articles

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How to Cite

1.
Association of Glutathione S-transferase (GSTM1, GSTT1 and GSTP1) polymorphisms and passive smoking in cervical cancer cases from North India. Int Jour of Biomed Res [Internet]. 2013 Dec. 30 [cited 2026 Mar. 9];4(12):655-62. Available from: https://ssjournals.co.in/index.php/ijbr/article/view/905