Analytical method development and validation of Doxazosin Mesylate uncoated tablets by RP-HPLC

Authors

  • M. R. Jayapal Institute of Aeronautical Engineering College Hyd, Affiliated to (JNTU-Hyderabad)
  • Santosh Kumar A Sreevatsav MRR College Of Pharmacy, Nadergul, Saroornagar, R.R.Dist
  • Shyam Sunder K Dept. of Pharmaceutical Chemistry, MLR Institute of Pharmacy, Dundigal (V), Hyderabad-43, A P
  • Harish Racha Dept. of Pharmaceutical Chemistry, MLR Institute of Pharmacy, Dundigal (V), Hyderabad-43, A P

DOI:

https://doi.org/10.7439/ijbr.v4i9.306

Abstract

A Simple, Precise, Stability indicating reverse phase HPLC method has been developed and validated in order to determine Doxazosin mesylate uncoated tablet dosage form. A Chromolith RP-18 column made up of Stainless Steel has been used with dimensions 100mm x 4.6mm x 10m. An Isocratic method with Mobile phase consisting of phosphate buffer: Methanol (40:60) ratio. A UV-Detector is used and the wavelength was set at 251nm.At the flow rate of 1.0ml/min the mobile phase was run for 10 minutes. The method was validated for Linearity, Precision, Accuracy, Robustness, and Stability of analytical solution. The Linearity range for the standard and the placebo was found to be between 50% and 150%. The method was validated for precision in method, system and intermediate. All the validation parameters have come under the limits.

Downloads

Download data is not yet available.

Downloads

Published

2013-09-30

Issue

Vol. 4 No. 9 (2013): Sep

Section

Original Research Articles

License

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
  • An author must submit Copyright form After acceptance of the article.

How to Cite

1.
Analytical method development and validation of Doxazosin Mesylate uncoated tablets by RP-HPLC. Int Jour of Biomed Res [Internet]. 2013 Sep. 30 [cited 2026 Apr. 25];4(9):465-76. Available from: https://ssjournals.co.in/index.php/ijbr/article/view/870