Antimicrobial activity of traditionally used herbs against human pathogens

Authors

  • Shweta Sen Thalwal Ambala College of Engineering and Applied Research, Ambala Cantt, Haryana 133101
  • Amit Gupta Department of Biotechnology Engineering, Ambala College of Engineering & Applied Research, Devsthali, Mithapur (Ambala), Haryana
  • Niyati Saini Department of Biotechnology Engineering, Ambala College of Engineering & Applied Research, Devsthali, Mithapur (Ambala), Haryana
  • Shailender Kumar Patel Department of Biotechnology Engineering, Ambala College of Engineering & Applied Research, Devsthali, Mithapur (Ambala), Haryana
  • Lalit Kapoor Department of Biotechnology Engineering, Ambala College of Engineering & Applied Research, Devsthali, Mithapur (Ambala), Haryana

DOI:

https://doi.org/10.7439/ijbr.v4i6.271

Keywords:

Salmonella, Shigella, Diarrhea, HIV/AIDS, CD4 count, Antiretroviral drugs.

Abstract

Antimicrobial agents are effective in curing diseases because of their selective toxicity against pathogenic microbes without causing any harm to the cells of the host. Antimicrobial activity of three herbs Amaranthus spinosus, Solanum nigrum and Orchis muscula were tested using methanol, hexane, chloroform and acetone extracts against six human pathogenic microbes. Agar well diffusion method was used for preliminary screening of extracts against microbes. The micro-dilution method was used for the determination of the minimal inhibition concentration (MIC). Phytochemical screening of plant extracts was done for the presence of various secondary metabolites. The results were analysed by using zone of inhibition and it was observed that methanol extracts of Orchis muscula and Amarnathus spinosus were effective against all the human pathogens tested.

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Author Biography

  • Shweta Sen Thalwal, Ambala College of Engineering and Applied Research, Ambala Cantt, Haryana 133101
    Assistant ProfessorDeptt of Biotechnology Engg & AR

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Published

2013-06-30

Issue

Vol. 4 No. 6 (2013): Jun

Section

Original Research Articles

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How to Cite

1.
Antimicrobial activity of traditionally used herbs against human pathogens. Int Jour of Biomed Res [Internet]. 2013 Jun. 30 [cited 2026 Mar. 8];4(6):236-40. Available from: https://ssjournals.co.in/index.php/ijbr/article/view/830