ISOLATION AND HPTLC ESTIMATION OF KAEMPFEROL FROM OXYSTELMA ESCULENTUM
DOI:
https://doi.org/10.7439/ijbr.v2i7.125Keywords:
Asclepiadaceae, HPTLC, KaempferolAbstract
Objectives: Oxystelma esculentum R. Br. (Family: Asclepiadaceae) is a perennial twiner growing in water-logged areas of the Indian sub-continent. It is used traditionally as diuretic, laxative and anti-ulcer. The present work deals with the isolation and structure elucidation of Kaempferol from O. esculentum and HPTLC method development for its estimation.
Methods: The petroleum ether extract of the entire plant of O. esculentum was subjected to further fractionation followed by pilot TLC experiments and column chromatography.
Results: This yielded a pure, yellow, crystalline solid which resolved at Rf 0.70 upon TLC of methanol fraction of the petroleum ether extract using the mobile phase toluene: methanol (3.5:6.5). This compound was subjected to UV, IR and 1H-NMR spectral analysis and its structure elucidation revealed it to be Kaempferol. A novel HPTLC method for the estimation of Kaempferol from O. esculentum was developed, which was found to be 0.879 ± 0.05%w/w.
Conclusion: The method developed was found to be easy, simple, precise, efficient, accurate, reproducible, specific and sensitive, and could serve as a suitable tool for routine analysis and phytochemical authentication of O. esculentum.
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