METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF CLOSANTEL IN TABLET DOSAGE FORM BY RP-HPLC METHOD

Authors

  • Gayatri S. Lecturer, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Sri Ramachandra University, Porur,Chennai, Tamil Nadu-600116
  • Mythili K. Lecturer, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Sri Ramachandra University, Porur,Chennai, Tamil Nadu-600116
  • Geethanjali M. Lecturer, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Sri Ramachandra University, Porur,Chennai, Tamil Nadu-600116
  • Chitra K. Lecturer, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Sri Ramachandra University, Porur,Chennai, Tamil Nadu-600116
  • C.Umamaheswara Reddy Lecturer, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Sri Ramachandra University, Porur,Chennai, Tamil Nadu-600116

DOI:

https://doi.org/10.7439/ijbr.v2i4.101

Keywords:

Closantel

Abstract

A rapid, sensitive and specific RP-HPLC method involving UV detection was developed and validated for determination of closantel in bulk and tablet dosage form. Chromatography was carried out on a phenomenex ODS C-18 column (250 x 4.6 mm), 5 μ particle size using filtered and degassed mixture of water pH adjusted to 3 and acetonitrile in the ratio 10:90 as mobile phase at a flow rate of 1.2 ml/min and effluent was monitored at 333 nm. The method was validated in terms of linearity, precision accuracy and specificity. The assay was linear over the concentration range of 100-500 μg/ml. Accuracy of the method was determined through recovery studies by adding known quantities of standard drug to the pre analysed test solution and was found to be 99.53% - 101.06% within precision RSD of 0.00502 for closantel. The system suitability parameters such as theoretical plates, retention time and tailing factor were found to be 10832, 6, and 1.27 respectively. The method does require only 10 mins as run time for analysis, which proves the adoptability of the method for the routine quality control of the drug.

Downloads

Download data is not yet available.

Downloads

Published

2011-05-01

Issue

Vol. 2 No. 4 (2011): Apr

Section

Original Research Articles

License

Copyright (c) 2011 International Journal of Biomedical Research

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
  • An author must submit Copyright form After acceptance of the article.

How to Cite

1.
METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF CLOSANTEL IN TABLET DOSAGE FORM BY RP-HPLC METHOD. Int Jour of Biomed Res [Internet]. 2011 May 1 [cited 2026 Mar. 8];2(4):246-51. Available from: https://ssjournals.co.in/index.php/ijbr/article/view/655