A HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR QUANTIFICATION OF TRANDOLAPRIL USING UV SPECTROMETRIC DETECTION

Authors

  • Sunil Kumar Dubey Pharmacy Group, Birla Institute of Technology and Science, Pilani-333 031
  • Swapnil Deshpande Pharmacy Group, Birla Institute of Technology and Science, Pilani-333 031
  • Sandeep Kumar Pharmacy Group, Birla Institute of Technology and Science, Pilani-333 031
  • Prashant Raut Pharmacy Group, Birla Institute of Technology and Science, Pilani-333 031
  • Akash Kumar Jain Pharmacy Group, Birla Institute of Technology and Science, Pilani-333 031
  • Rajeev J. Mudakavi Pharmacy Group, Birla Institute of Technology and Science, Pilani-333 031

DOI:

https://doi.org/10.7439/ijbr.v1i3.66

Keywords:

Post traumatic hydrocephalus, post head injury hydrocephalus, v.p shunting

Abstract

A high performance Liquid chromatographic method for quantification of trandolapril using UV detection was developed and validated. Trandolapril samples were analysed on Merck LiChroCART -RP C18 column (250x4.0, i.d.5 μm) and the mobile phase composition used for detection was combination of acetonitrile: methanol: phosphate buffer (0.025mM) pH3.0 (40:35:25)at a flow rate of 1 ml/min. The λmax used was 220nm with UV detection. The retention time of trandolapril by proposed method was found to be 2.750 ± 0.008 min. Peak area obtained were linearly related to concentration of drug in samples in range of 2.5-17.5 μg/mL having correlation coefficient of 0.999. The LOD and LOQ of trandolapril by proposed method was found to be 0.099 μg/mL and 0.300834 μg/ml respectively. The method was validated as per ICH guidelines for various parameters. The results for accuracy, precision and robustness were found to be within accepted limits.

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Published

2010-11-01

Issue

Vol. 1 No. 3 (2010): Oct

Section

Original Research Articles

License

Copyright (c) 2010 International Journal of Biomedical Research

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How to Cite

1.
A HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR QUANTIFICATION OF TRANDOLAPRIL USING UV SPECTROMETRIC DETECTION. Int Jour of Biomed Res [Internet]. 2010 Nov. 1 [cited 2026 Mar. 9];1(3):133-40. Available from: https://ssjournals.co.in/index.php/ijbr/article/view/622