Hibiscus sabdariffa shows the potential of downregulating the expression of the Kiss1 gene in the ovary of Wistar rats.

Authors

  • Izuchukwu Azuka Okafor Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, PMB 5001, Nnewi, Nigeria. Department of Obstetrics and Gynaecology, College of Medicine, University of Ibadan, Ibadan, Nigeria. Pan African University of Life and Earth Science Institute (Including Health and Agriculture), PAULESI, University of Ibadan, Ibadan, Nigeria. https://orcid.org/0000-0003-1017-9297
  • Uchenna Somtochukwu Nnamah Hematology Department, Babcock University Teaching Hospital, Ilisan-Remo, Ogun State, Nigeria
  • Johnson Okwudili Nweke Diagnostic Laboratory Unit, Medical Centre, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria https://orcid.org/0000-0002-5592-4978
  • Jude Amechi Nnaka Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, PMB 5001, Nnewi, Nigeria.
  • Selasie Ahiatrogah Department of Obstetrics and Gynaecology, College of Medicine, University of Ibadan, Ibadan, Nigeria. Pan African University of Life and Earth Science Institute (Including Health and Agriculture), PAULESI, University of Ibadan, Ibadan, Nigeria https://orcid.org/0000-0001-9732-8309
  • Uche Valentine Okeke Morbid Anatomy Department, Alex Ekwueme Federal University Teaching Hospital Abakaliki, Ebonyi State, Nigeria

Keywords:

roselle, ovary, kiss1 gene, gene expression, hormones, antioxidant, histology, Hibiscus sabdariffa

Abstract

Objectives: This study investigated the Kiss1 gene expression in the ovary, following the administration of Methanolic extract of Hibiscus sabdariffa (MEHS) in Wistar rats.

Methods: Fifteen (15) rats with an average weight of 148 g were randomly divided into three (3) groups (n=5), A-C. Group A was given no treatment and served as the normal control group. Groups B and C received oral administration of 200mg/kg and 400mg/kg of MEHS respectively. The extract was administered once a day for 21 days.

Result: No significant difference was observed in the relative ovarian weight, reproductive hormone levels, and ovarian antioxidant levels in all the treatment groups compared to the control (p>0.05). There is a significantly lower intensity of expression of the Kiss1 gene in the ovarian tissue of group C (p=0.011) when compared to the control group. There is no significant correlation between the relative intensity of Kiss1 gene expression in the ovary and the reproductive hormones of all the experimental groups (p =.879, .534, .133; r=.081, .322, -.686). MEHS caused no histopathological changes in the ovary at both treatment doses (200mg/kg and 400mg/kg).

Conclusion: MEHS shows the potential of downregulating the expression of the Kiss1 gene in the ovary, albeit at a higher dose. However, this effect lacks a regulatory mechanism on the reproductive hormones and ovarian antioxidative levels.

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Published

2020-11-30

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Section

Original Research Articles

How to Cite

1.
Hibiscus sabdariffa shows the potential of downregulating the expression of the Kiss1 gene in the ovary of Wistar rats. Int Jour of Biomed Res [Internet]. 2020 Nov. 30 [cited 2025 Jul. 25];11(11):e5535. Available from: https://ssjournals.co.in/index.php/ijbr/article/view/5535