Study of Human papillomavirus (HPV) - Related Oropharyngeal Squamous Cell Carcinoma as a distinct entity in Head and Neck Squamous Cell Carcinoma (HNSCC)
Keywords:
Human papillomavirus, Head and neck squamous cell carcinoma, Histomorphological, p16 IHC, HPV-DNA in situ hybridizationAbstract
Background: Human papillomavirus (HPV) associated head and neck squamous cell carcinoma (HNSCC) is an entity with unique clinical and molecular characteristics. The detection of HPV is clinically important and for that several methods are currently available. Aim of the present study was to determine the histomorphological features of HPV-related oropharyngealsquamous cell carcinomas (OPSCC) and to detect HPV using p16 IHC staining and HPV DNA in situ hybridization/IHC.
Method: A total 17 cases of HNSCC were studied during a period from 1st January 2013 to 30th June 2014. Tumors with clinical and histopathological features of HPV related HNSCC were tested for HPV by p16 IHC staining and HPV DNA In situ hybridisation/IHC.
Results: Among 17 patients, 13(66.5%) had conventional SCC and 4(23.5%) patients had HPV-related OPSCC.Most cases of HPV-related OPSCC were in 6th decade (M: F= 1:1) while majority of conventional SCC were in 7th decades with male predominance (61.53%). Tonsil & palate (75%) was the commonest site of tumor in cases of HPV-related OPSCC while in conventional SCC, buccal mucosa, tongue, gingiva, pharynx, and larynx were the commonest site of tumor (92.30%). 2 cases of HPV-related OPSCC had history of tobacco addiction as compared to 8 in conventional SCC. In HPV-related OPSCC histopathological figures were non-keratinizing tumor with tumor infiltrating lymphocytes while the features of conventional SCC were of keratinizing SCC infiltrating in cords and nests with desmoplasia and surface dysplasia.
Conclusion: HPV related HNSCC has a distinct histomorphology and clinical presentation. p16 and ISH are useful HPV detection methods in fixed tissue.
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