A study of hVISA among Methicillin Resistant Staphylococcus aureus isolates in a tertiary care hospital in Central India.
DOI:
https://doi.org/10.7439/ijbr.v10i5.5174Keywords:
S. aureus, Heterogeneous VISA, Vancomycin, Minimum Inhibitory ConcentrationAbstract
Introduction: Since the first stain of S. aureus with reduced susceptibility to vancomycin was reported from Japan, there has been an increase in the number of cases with both VISA and VRSA (Vancomycin-Intermediate and Vancomycin-Resistant S. aureus respectively). hVISA (heterogenous Vancomycin-Intermediate S. aureus) strains are phenotypically susceptible to vancomycin by routine laboratory methods but contain vancomycin-intermediate subpopulations on culture. Heterogeneous VISA strains are responsible for clinical failures to vancomycin treatment of otherwise apparently susceptible S. aureus strains.4
Materials and methods: A total of 287 Staphylococcus aureus clinical isolates were included in study. In-house vancomycin screen agar plate was prepared by addition of 6 mg/l vancomycin (Hi- media laboratories Pvt, Ltd. Mumbai) to brain heart infusion (BHI) agar. MIC (Minimum Inhibitory Concentration) of vancomycin was performed by E strip method. 15ug vancomycin disc was prepared in-house using pure drug procured from Hi media with code no CMS217 for detection of hVISA.
Results: Out of total 287 Staphylococcus aureus clinical isolates, 46.68% were found to be MRSA (Methicillin resistant S. aureus). A total of 8 isolates were detected by Vancomycin screen agar for screening hVISA. Detection of hVISA by 15
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