Antioxidant Enzyme Activities (Superoxide Dismutase and Catalase) in Peripheral Blood Mononuclear Cells in Essential Hypertension

Authors

  • Gauri Pathare Sir H.N. Medical Research Society, Sir H.N. Reliance Foundation Hospital and Research Center
  • Sunila Raju Research Assistant,, Sir H.N. Medical Research Society, Sir H.N. Reliance Foundation Hospital and Research Center
  • Manoj Mashru Department of Cardiology, Sir H.N. Reliance Foundation Hospital and Research Center, Raja Ram Mohan Roy Road, Mumbai 400004
  • Vinod Shah Department of Cardiology, Sir H.N. Reliance Foundation Hospital and Research Center, Raja Ram Mohan Roy Road, Mumbai 400004
  • Kavita Shalia Senior Scientist, Sir H.N. Medical Research Society, Sir H.N. Reliance Foundation Hospital and Research Center, Court House, L. T. Road, Mumbai 400002

DOI:

https://doi.org/10.7439/ijbr.v10i1.4990

Abstract

Background: Oxidative stress has been implicated in pathogenesis of hypertension, a major cardiovascular risk factor. Peripheral blood mononuclear cells (PBMCs) are primary contributors to systemic reactive oxygen species (ROS). The impact of ROS-products released from PBMCs may contribute to endothelial dysfunction damage in hypertensive subjects. Superoxide Dismutases (SODs) and Catalase comprise first line of defence against ROS. Aim: To analyse activities of SOD and Catalase in PBMCs of freshly diagnosed essential hypertensive patients as compared to normotensive healthy controls. Further, correlate the antioxidant enzyme activities with oxidative stress in terms of 8-iso-Prostaglandin F2α (8-iso-PGF2α) in both groups. Methods: Forty-eight patients and age, BMI-matched 48 controls were recruited. Catalase and SOD activities were measured in PBMC-lysates using Enzyme-Linked-Immunosorbent-Assay (ELISA). 8-iso-PGF2α levels in serum and urine of all subjects were also measured using ELISA. Results: Significantly lower (5.1-fold, p 0.001) Catalase activity was found in patients as compared to controls. Similar but non-significant trend (1.3-fold) was observed for SOD.8-iso-PGF2α levels were significantly higher in serum (11.3-fold, p 0.001) and urine (4.8-fold, p 0.001) of patients as compared to controls. In patients, activity of SOD showed positive correlation with that of Catalase (rs=0.383, p=0.007) and serum 8-iso-PGF2α levels (rs=0.459, p=0.001). While in controls, Catalase activity correlated negatively with 8-iso-PGF2α (rs=-0.344, p=0.017). Conclusion: This study reports significantly increased oxidative stress in hypertensive patients accompanied by a simultaneous decrease in the antioxidant enzyme activities of Catalase and SOD in their PBMCs. A direct correlation between SOD activity and oxidative stress suggests its defensive position while Catalase seems to adopt a submissive role in defence system in case of essential hypertension.

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Author Biographies

  • Gauri Pathare, Sir H.N. Medical Research Society, Sir H.N. Reliance Foundation Hospital and Research Center

    Ph.D. Fellow

  • Manoj Mashru, Department of Cardiology, Sir H.N. Reliance Foundation Hospital and Research Center, Raja Ram Mohan Roy Road, Mumbai 400004

    MD (Med), DM (Card)

  • Vinod Shah, Department of Cardiology, Sir H.N. Reliance Foundation Hospital and Research Center, Raja Ram Mohan Roy Road, Mumbai 400004

    DM (Card.), MD (Med.), FISE

  • Kavita Shalia, Senior Scientist, Sir H.N. Medical Research Society, Sir H.N. Reliance Foundation Hospital and Research Center, Court House, L. T. Road, Mumbai 400002

    Ph.D.

References

Gonzalez J, Valls N, Brito R, Rodrigo R. Essential hypertension and oxidative stress: New insights. World J Cardiol 2014; 6(6): 353-66.

Beg M, Gupta A, Khanna VN. Oxidative Stress in Essential Hypertension and Role of Antioxidants. JIACM 2010; 11(4): 287-93.

Lassegue B, Griendling KK. Reactive oxygen species in hypertension; An update. Am J Hypertens 2004; 17(9): 852-60.

Rodrigo R, Gonzalez J, Paoletto F. The role of oxidative stress in the pathophysiology of hypertension. Hypertens Res 2011; 34(4): 431-40.

Birben E, Sahiner UM, Sackesen C, Erzurum S, Kalayci O. Oxidative stress and antioxidant defense. World Allergy Organ J 2012; 5(1): 9-19.

Deo SH, Holwerda SW, Keller DM, Fadel PJ. Elevated peripheral blood mononuclear cell-derived superoxide production in healthy young black men. Am J Physiol Heart Circ Physiol 2015; 308(5): H548-52.

Redon J, Oliva MR, Tormos C, Giner V, Chaves J, Iradi A, et al. Antioxidant activities and oxidative stress byproducts in human hypertension. Hypertension 2003; 41(5): 1096-101.

Devasagayam TP, Boloor KK, Ramasarma T. Methods for estimating lipid peroxidation: an analysis of merits and demerits. Indian J Biochem Biophys 2003; 40(5): 300-8.

Montuschi P, Barnes PJ, Roberts LJ 2nd. Isoprostanes: markers and mediators of oxidative stress. FASEB J 2004; 18(15): 1791-800.

Jacob KD, Noren Hooten N, Trzeciak AR, Evans MK. Markers of oxidant stress that are clinically relevant in aging and age-related disease. Mech Ageing Dev 2013; 134(3-4): 139-57.

Milne GL, Musiek ES, Morrow JD. F2-isoprostanes as markers of oxidative stress in vivo: an overview. Biomarkers 2005; 10 Suppl 1: S10-23.

Morrow JD, Hill KE, Burk RF, Nammour TM, Badr KF, Roberts LJ 2nd. A series of prostaglandin F2-like compounds are produced in vivo in humans by a non-cyclooxygenase, free radical-catalyzed mechanism. Proc Natl Acad Sci U S A 1990; 87(23): 9383-7.

Chobanian AV, Bakris GL, Black HR, Cushman WC, Green LA, Izzo JL Jr, et al. The Seventh Report of the Joint National Committee on Prevention, Detection, Evaluation, and Treatment of High Blood Pressure: the JNC 7 report. JAMA 2003; 289(19): 2560-72.

Russo C, Olivieri O, Girelli D, Faccini G, Zenari ML, Lombardi S, et al. Anti-oxidant status and lipid peroxidation in patients with essential hypertension. J Hypertens 1998; 16(9): 1267-71.

Venkatesan B, Mahimainathan L, Das F, Ghosh-Choudhury N, Ghosh Choudhury G. Downregulation of catalase by reactive oxygen species via PI 3 kinase/Akt signaling in mesangial cells. J Cell Physiol 2007; 211(2): 457-67.

Otitoju O, Onwurah IN, Otitoju GT, Ugwu CE. Oxidative stress and superoxide dismutase activity in brain of rats fed with diet containing Permethrin. Biokemistri 2008; 20: 93–8.

Cracowski JL, Cracowski C, Bessard G, Pepin JL, Bessard J, Schwebel C, et al. Increased lipid peroxidation in patients with pulmonary hypertension. Am J RespirCrit Care Med 2001; 164(6): 1038-42.

Zhang R, Sun ML, Fan YF, Jiang X, Zhao QH, He J, et al. Plasma 15-F2t-isoprostane in idiopathic pulmonary arterial hypertension. Int J Cardiol 2014; 175(2): 268-73.

Hozawa A, Ebihara S, Ohmori K, Kuriyama S, Ugajin T, Koizumi Y, et al. Increased plasma 8-isoprostane levels in hypertensive subjects: the Tsurugaya Project. Hypertens Res 2004; 27(8): 557-61.

Belch JJ, Bridges AB, Scott N, Chopra M. Oxygen free radicals and congestive heart failure. Br Heart J 1991; 65(5): 245-8.

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Published

2019-01-23

Issue

Vol. 10 No. 1 (2019): Jan

Section

Original Research Articles

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How to Cite

1.
Antioxidant Enzyme Activities (Superoxide Dismutase and Catalase) in Peripheral Blood Mononuclear Cells in Essential Hypertension. Int Jour of Biomed Res [Internet]. 2019 Jan. 23 [cited 2025 Mar. 12];10(1):e4990. Available from: https://ssjournals.co.in/index.php/ijbr/article/view/4990