Convenient and efficient method for ingestion of L-NAME to induce hypertension in rats: An alternative to oral gavage

Authors

  • Ankit Gupta Resident, Department of Physiology, KGMU. Lucknow
  • Pradeep Kumar Professor,Physiology Department,KGMU Lucknow,India
  • Sunita Tiwari Professor and Head,Physiology Department,KGMU Lucknow,India
  • Rajendra Nath Professor,Pharmacology Department,KGMU Lucknow,India

DOI:

https://doi.org/10.7439/ijbr.v9i10.4930

Abstract

Objectives : Administration of drug by oral route is used since a long time in Indian traditional medicine and experimental procedures. Oral gavage is one of the best methods to deliver drug to animals, but it need skilled hand with various harmful effects. So an alternative to oral gavage is warranted now a day. Materials and Methods: The study was conducted on twelve male wistar rats randomly divided into two groups: Group A (n=6) and Group B (n=6). L-NAME was given orally to animals of group B for 21 days daily in the morning time in a dosage of 40mg/kg body weight to induce hypertension. L-NAME was mixed in special custom made food (vehicle) which was highly acceptable and easily consumed by rats which deliver L-NAME in full required dose. Body weight, systolic blood pressure and heart rate were recorded and analyzed before and after giving L-NAME. Results : Systolic blood pressure was increased from 120.95

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Author Biographies

  • Pradeep Kumar, Professor,Physiology Department,KGMU Lucknow,India

    Professor,Physiology Department,KGMU Lucknow,India

  • Sunita Tiwari, Professor and Head,Physiology Department,KGMU Lucknow,India

    Professor and Head,Physiology Department,KGMU Lucknow,India

  • Rajendra Nath, Professor,Pharmacology Department,KGMU Lucknow,India

    Professor,Pharmacology Department,KGMU Lucknow,India

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Published

2018-10-28

Issue

Vol. 9 No. 10 (2018): Oct

Section

Original Research Articles

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How to Cite

1.
Convenient and efficient method for ingestion of L-NAME to induce hypertension in rats: An alternative to oral gavage. Int Jour of Biomed Res [Internet]. 2018 Oct. 28 [cited 2025 Mar. 12];9(10):346-8. Available from: https://ssjournals.co.in/index.php/ijbr/article/view/4930