Diagnostic utility of Plasma Thromboplastin cell block preparation in cytological evaluation of serous effusions
DOI:
https://doi.org/10.7439/ijbr.v6i11.2712Abstract
Background: Cell block preparations act as a useful adjunct to smear cytology for categorization of malignant and benign effusions. Plasma thromboplastin cell block technique is simple, requires less time and offers improved cytomorphological features. Aim: To study the utility of Thromboplastin-plasma cell block technique and its diagnostic significance in conjunction with conventional cytology smears in evaluation of serous effusions. Material and methods : One hundred samples were included in the study. In addition to preparation of conventional smears, fluids were subjected to cell block technique. Cell blocks were prepared using Plasma thromboplastin cell block technique. Results : Cellularity and diagnostic yield for malignancy was increased by cell block preparation. Conclusion : Plasma Thromboplastin cell block method provides high cellularity, better architectural patterns and good preservation of cellular and nuclear details, thereby, increasing diagnostic yield in the cytological evaluation of serous effusions when compared to conventional smears alone. Moreover, it is easier to apply immunomarkers and perform molecular studies on the cell blocks that can be stored indefinitely for future testing. Thus, cell blocks can act as a useful adjunct to the conventional cytosmears for evaluation of serous effusions.Downloads
References
Shivakumarswamy U, Arakeri SU, Karigowdar MH, Yelikar B R. Diagnostic utility of the cell block method versus the conventional smear study in pleural fluid cytology. J Cytol 2012; 29:11-5.
Thapar M, Mishra RK, Sharma A, Goyal V, Goyal V. Critical analysis of cell block versus smear examination in effusions. J Cytol 2009; 26:60-4.
Nathan NA, Narayan E, Smith MM, Horn MJ. Cell blocks cytology. Improved preparation and its efficacy in diagnostic cytology. Am J Clin Pathol. 2000; 114:599-606.
Leung SW, Bedard YC. Methods in pathology: simple miniblock technique for cytology. Mod Pathol. 1993; 6:630-632.
Keyhani-Rofaga S, O
Kulkarni MB, Desai SB, Ajit D, Chinoy RF. Utility of the thromboplastin-plasma cell-block technique for fine-needle aspiration and serous effusions. Diagn Cytopathol. 2009; 37(2):86-90.
Mair, Dunbar F, Becker PJ, DuPlessis W. Fine Needle Cytology: Is aspiration suction necessary? A study of 100 masses in various sites. ActaCytol 1989; 33:809-13.
Bahrenburg LPH. On the diagnostic results of the microscopical examination of the ascitic fluid in two cases of carcinoma involving the peritoneum. Cleveland Med Gaz.1896; 11:274-278.
Shidham VB, Epple J. Appendix I: Collection and processing of effusion fluids for cytopathologic evaluation. In: Shidham VB, Atkinson BF, (edi). Cytopathologic Diagnosis of Serous Fluids. 1st edn. London: Elsevier; 2007. p. 207-35.
Steven A Hecht, Matthew McCormack. Comparison of three cell block techniques for detection of low frequency abnormal cells. Dovepress 2013; 5:1-7.
Nigro K, Tynski Z, Wasman J, Abdul-Karim F, Wang N. Comparison of cell block preparation methods for nongynecologic ThinPrep specimens. Diagn Cytopathol. 2007; 35:640
Crapanzano JP, Heymann JJ, Monaco S, Nassar A, Saqi A. The state of cell blocks variation and satisfaction in the era of molecular diagnostics and personalized medicine. CytoJournal 2014; 11:7.
Bhanvadia VM, Santwani PM, Vacbhani JH. Analysis of diagnostic value of cytological smear method versus cell block method in body fluid cytology: study of 150 cases. Ethiop J Health Sci 2014; 24:2.
Kushwaha R, Shashikala P, Hiremath S, Basavaraj HG. Cells in pleural fluid and their value in differential diagnosis. J Cytol 2008; 25:138-43.
Singh M, Khan L, Verma YN, Sachan N, Pantola C, Pathak A, et al. Comparative study for the use of different techniques in serous fluid cytology. Journal of evolution of medical and dental sciences 2015; 4(18): 3154-3161.
Shubhada B, Kumbalkar D, Nayak S. Evaluation of Cell Block Technique in the Cytodiagnosis of Body Fluids. International Journal of Science and Research 2015; 4 (7): 87-94.
Richardson HL, Koss LG, Simon TR. Evaluation of concomitant use of cytological and histological technique in recognition of cancer in exfoliated material from various sources. Cancer 1955; 8:948-50.
Dekker A, Bupp PA. Cytology of serous effusions. An investigation in to the Usefulness of Cell Block versus Smears. Am J Clin Pathol 1978; 70:855- 60.
Khan N, Sherwani KR. Usefulness of cell blocks versus smears in malignant effusion cases. J Cytol 2006; 23:129-32.
Fetsch PA, Abati A. Immunocytochemistry in effusion cytology. Cancer 2001; 93:293
Sakamoto H., Takenaka M., Ushimaru K., Tanaka T.. Use of Liquid-Based Cytology (LBC) and Cell Blocks from Cell Remnants for Cytologic, Immunohistochemical, and Immunocytochemical Diagnosis of Malignancy. Open Journal of Pathology 2012; 2(3): 58-65.
Flens MJ, Valk PVD, Tadema TM, Huysmans ACLM, Risse EKJ, Van Tol GA, et al. The contribution of immunocytochemistry in diagnostic cytology. Comparison and evaluation with immunohistology. Cancer 1990; 65: 2704
Gong Y, Sun X, Michael CW, Attal S, Williamson BA, Bedrossian CW. Immunocytochemistry of serous effusion specimens: a comparison of Thin Prep vs cell block. Diagn Cytopathol. 2003; 28(1): 1-5.
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