Effectiveness of Haemagglutination Test in Detection of Newcastle Disease Virus (NDV) AF2240 Strain from Harvested Allantoic Fluids of Infected Chicken Eggs

Authors

  • Umar Ahmad Research Laboratory of Anatomy and Histology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor Malaysia
  • Ismaila Ahmed Research Laboratory of Anatomy and Histology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor Malaysia
  • Fauziah Othma Department of Human Anatomy, Faculty of Medicine and Health Sciences, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia,
  • Young Yoke Keong Department of Human Anatomy, Faculty of Medicine and Health Sciences, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia,

DOI:

https://doi.org/10.7439/ijbr.v5i10.751

Abstract

Haemagglutination (HA) test for the detection of Newcastle disease virus (NDV) is described experimentally using chicken red blood cells (RBCs). The method proved to be reliable and effective, since infectious stocks of AF2240 strains of NDV were detected after the experimental procedure. To detect NDV in chicken allantoic fluids of SPF eggs, 0.1 mL of NDV AF2240 (velogenic) virus stock were inoculated into each egg. The virus was allowed to propagate for production of more stock and the unclarified allantoic fluids were harvested aseptically and tested for confirmation of the virus presence before the stock virus was further clarified and purified using high speed refrigerated centrifuge following sucrose gradients method. This sucrose gradients were later balanced with NTE buffer and sieved using 0.45 m filter and the purified samples of the virus were collected followed by HA test to detect the presence and to determine the virus titer. The results showed (28 = 256 HAU/mL) virus titer after purification of the allantoic fluids.

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Author Biographies

  • Umar Ahmad, Research Laboratory of Anatomy and Histology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor Malaysia
    Postgratduate Research Student, Of the Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia.
  • Ismaila Ahmed, Research Laboratory of Anatomy and Histology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor Malaysia
    Postgraduate Student
  • Fauziah Othma, Department of Human Anatomy, Faculty of Medicine and Health Sciences, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia,
    Professor, Head of department of human anatomy
  • Young Yoke Keong, Department of Human Anatomy, Faculty of Medicine and Health Sciences, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia,
    Senior lecturer, Department of Human Anatomy

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Published

2014-10-20

Issue

Vol. 5 No. 10 (2014): Oct

Section

Original Research Articles

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How to Cite

1.
Effectiveness of Haemagglutination Test in Detection of Newcastle Disease Virus (NDV) AF2240 Strain from Harvested Allantoic Fluids of Infected Chicken Eggs. Int Jour of Biomed Res [Internet]. 2014 Oct. 20 [cited 2026 Feb. 16];5(10):606-9. Available from: https://ssjournals.co.in/index.php/ijbr/article/view/1083