Stem cells from apical papilla and their properties in two primary culture methods
DOI:
https://doi.org/10.7439/ijbr.v5i8.720Abstract
Background and Objective: Stem cells from the apical papilla (SCAP) are a unique population of dental mesenchymal stem cells and also different to dental pulp stem cells. Study and application of this cell type are extremely important in natural tooth root regeneration, especially in the treatment of oral diseases. Studies have isolated and characterized stem cell properties of SCAP by only one culture method- enzymatic dispersal method. The purpose of this study was to compare two primary culture methods in isolation and characterization of SCAP. Materials and Methods: The effects of two methods on the cell viability, colony-forming efficiency, proliferation rate and multilineage differentiation potential and profiles of mesenchymal stem cell markers in vitro have been examined. Result: We found that cultured cells adhered to the surface and developed with the same proliferation rate and formed colony units in both methods. In the enzymatic dispersal method, time for primary culture is shorter, differentiation potential is also higher than in outgrowth method. However, the cultured population of cells by outgrowth method is more homogenous with high expression of CD44, CD73, CD90 and negative with CD34, CD45, HLADR ( 2%). Conclusion: We conclude that two methods are available for primary culture of SCAP and in different situations, we should use more appropriate method.
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