Effects of blood components modulation on load-dependent cardiac function during blood exchange viewing from pressure-volume loops

Abstract

Changing of blood components, both red blood cells and plasma, particularly affects on the circulatory system. This study investigated the changing of load-dependent cardiac function viewing from pressure-volume loops after normovolemic exchange or hemodilution with three different transfusion fluids. The investigation was experimentally conducted in anesthetizedmale Golden Syrian hamsters. Twelve hamsters were randomly assigned into 4 groups. Normovolemic hemodilution was performed by the exchange of 40% of the animals blood volume with transfusion fluid. Three different transfusion fluids included 3-week stored blood, dextran 70 kDa (viscosity 3.0 cP) and high viscosity plasma volume expander (HVPE; 6% dextran 2000 kDa, viscosity 6.4 cP). One group was not performed any exchange to use as the control group. Left ventricular cardiac systolic function was quantitatively evaluated with a pressure-volume conductance catheter. A pressure-volume loop transition was noticeable in all groups. Cardiac output at the end of experiment increased from baseline in the group hemodiluted with HVPE and dextran 70 kDa. On the other hand, cardiac output in the group exchanged with stored blood was less than baseline. Stroke work relative to baseline in the hemodiluted groups increased whereas it decreased in the group exchanged with stored blood. These data suggested that changing plasma viscosity increases load-dependent cardiac function while aged red blood cells reduces load-dependent cardiac function when compared to baseline.