Development of validated RP-HPLC method for the simultaneous estimation of atenolol and chlorthalidone in combine tablet dosage form

Authors

  • Manoj Charde Government College of Pharmacy, Kathora Naka, Amravati-444604, Maharashtra
  • A. S. Welankiwar Government College of Pharmacy, Kathora Naka, Amravati-444604, Maharashtra

DOI:

https://doi.org/10.7439/ijap.v3i1.1415

Abstract

A RP-HPLC method for the estimation of ATN (Atenolol) and CTN (chlorthalidone) in combined dosage form was developed using Comosil RP-C18 (4.6 x 250mm, 5m) in an gradient mode with mobile phase comprising of Methanol: Water (pH 3 using OPA) The flow rate was 1 mL/ min and effluent was monitored at 226.0 nm. The retention times were found to be 2.2 min for ATN and 3.36 min for CTN. The assay exhibited a linear dynamic range of 40- 200 g/mL for ATN and 10- 50 g/mL for CTN. The calibration curves were linear (r 2 = 0.999 for ATN and r 2 = 0.999 for CTN) over the entire linear range. Mean % recovery was found to be 99.78 % for ATN and 99.30 % for CTN with % RSD was NMT 2 for both estimations which fully agrees with system suitability which is in good agreement with labeled amount of formulation. The % RSD for Intra- Day and Inter-Day Precision was NMT than 2 for both the drugs. The developed method was validated as per ICH guidelines

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Published

2014-03-31

Issue

Vol. 3 No. 1 (2014): Jan-Feb

Section

Research Articles

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How to Cite

1.
Development of validated RP-HPLC method for the simultaneous estimation of atenolol and chlorthalidone in combine tablet dosage form. Int J Adv Pharm [Internet]. 2014 Mar. 31 [cited 2026 Jan. 3];3(1):06-18. Available from: https://ssjournals.co.in/index.php/ijap/article/view/1415
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