Oxidative stress and antimicrobial activity of formulated health drink

Abstract

A health drink was prepared by using various extracts of the fruits like sweet orange, grapes, Apple, carrot, banana. It was further evaluated for antioxidant activity, antimicrobial activity and stability studies. Physiochemical and Organoleptic parameter were evaluated like pH, viscosity, density, colour, odour and taste were also taken into consideration. In-vitro free radical scavenging activity by using beta-carotenes bleaching & lipid per oxidation method for the formulation was performed. The inhibition activity of the health drink on the peroxide ion of linoleic acid was measured by folic thiocyanate method in comparison to methanolic extract of green tea, ginkobiloba, vit.E, and BHA as positive controls. The antimicrobial activity was performed by Agar disk diffusion and Agar ditch diffusion methods using various strains of bacteria. Microbial growth was determined by measuring the diameter of the zone of inhibition. The health drink formulation of various fruits exhibited strong antioxidant activity and moderate antimicrobial activity. The health drink also showed promising stability studies.