Quality assessment and phytochemical analysis of Clerodendrum serratum roots
DOI:
https://doi.org/10.7439/ijpp.v6i3.3281Abstract
Objectives: Clerodendrum serratum (L.) Moon. (Verbenaceae) commonly known as bharangi is an important medicinal plant growing in India having traditional value to treat pain, inflammation, rheumatism, respiratory disorders and fever. The aim of present study is to develop pharmacognostical, physicochemical and phytochemical parameters for quality assessment of Clerodendrum serratum roots (CSR). Methods: The roots of C. serratum were evaluated for pharmacognostical, physicochemical parameters such as ash values (total ash, acid insoluble ash and water soluble ash), extractive values (alcohol and water soluble), moisture content, crude fiber content and foaming index according to WHO guidelines on quality control methods for medicinal plant materials. The phytochemical analysis was performed using chemical tests, ultraviolet spectroscopy, thin layer chromatography and quantitative estimation of secondary phytoconstituents. Results: The pharmacognostical and physicochemical parameters evaluated for the roots were found in compliance with the Pharmacopoeial standards of crude drug. The qualitative and quantitative phytochemical analysis of methanolic extract of CSR showed presence of phenolics, flavonoids, saponins and carbohydrates. Conclusion: The present investigation will be helpful for the identification and quality control of CSR.Downloads
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References
Patel JJ, Acharya SR, Acharya NS. Clerodendrum serratum (L.) Moon. - A review on traditional uses, phytochemistry and pharmacological activities. J Ethnopharmacol 2014; 154 (2):268-285.
Zalke AS, Kulkarni AV, Shirode DS, Duraiswamy B. In-vivo anticancer activity of Clerodendrum serratum (L) Moon. Res J Pharm BiolChemSci 2010; 1(3):89-98.
Vidya SM, Krishna V, Manjunatha BK, Mankani KL, Ahmed M, Singh SDJ. Evaluation of hepatoprotective activity of Clerodendrum serratum L. Indian J Exp Biol 2007; 45(6):538-542.
Narayanan N, Thirugnanasambantham P, Viswanathan S, Rajarajan S, Sukumar E. Comparative antibacterial activities of Clerodendrum serratum and Premna herbacea roots. ?Indian J Pharm Sci 2004; 66(4):453-454.
Narayanan N, Thirugnanasambantham P, Viswanathan S, Vijayasekaran V, Sukumar E. Antinociceptive, anti-inflammatory and antipyretic effects of ethanol extract of Clerodendrum serratum roots in experimental animals. J Ethnopharmacol 1999, 65(3):237-241.
Bhujbal SS, Kewatkar SMK, More LS, Patil MJ. Antioxidant effects of roots of Clerodendrum serratum Linn. Pharmacognosy Res 2009; 1(5):294-298.
Narayanan N, Thirugnanasambantham P, Viswanathan S, Sukumar E, Jeyaraman P. Pharmacognostical studies on the roots of Clerodendrum serratum. Pharm Biol 2002; 40(5):362-368.
Anonymous. World Health Organization (WHO), Quality Control Methods for Medicinal Plant Material. Geneva: Mondiale De Sante; 1998. p.40-53.
Kokate C K. Practical Pharmacognosy. 4th ed. Pune: Nirali Prakashan; 2000. p.107-111.
Mukherjee PK. Quality Control of Herbal Drugs. 1st ed. New Delhi: Business Horizon Pharmaceutical Publications; 2002. p.131-219.
Xiao JH, Xiao DM, Chen DX, Xiao Y, Liang ZQ, Zhong JJ. Polysaccharides from the medicinal mushroom Cordyceps taii show antioxidant and immunoenhancing activities in a D-galactose-induced aging mouse model. Evid Based Complementary Altern Med 2012; 2012:273-435.
Wagner H, Baldt S, Zgainski EM. Plant drug analysis. Berlin: Springer; 1996. p.195-308.
Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. ?Am J Enol Vitic1965; 16:144-158.
Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 1999; 64(4):555-559.
Hiai S, Oura H, Nakajima T. Color reaction of some sapogenins and saponins with vanillin and sulphuric acid. Planta Med 1976; 29(2):116-122.
Pawar HA, D
Nawagish M, Ansari SH, Ahmad A. Preliminary pharmacognostical standardization of Lawsonia inermis Linn. Seeds. Res J Botany 2007; 2(3):161-164.
Vijayalakshmi R, Ravindhran R. Comparative fingerprint and extraction yield of Diospyrus ferrea (willd.) Bakh. root with phenol compounds (gallic acid), as determined by UV-VIS and FT-IR spectroscopy. Asian Pac J Trop Biomed 2012; 2 Suppl 3:S1367-S1371.
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