Study of betacyanin level from cactus fruit (Opuntia elatior Mill) in Palu city using spectrophotometry UV-VIS

Authors

  • Yonelian Yuyun Department of Pharmacy Faculty of Mathematics and Natural Sciences Tadulako University, PALU

DOI:

https://doi.org/10.7439/ijpp.v6i4.3085

Abstract

A research has been done about comparative study of the levels of total anthocyanin fruit cactus ( Opuntia elatior Mill) from Palu city using UV-Visible spectrophotometry in order to determine the levels of total betasianin cactus fruit that comes from the Mantikulore, East Palu and South Palu Subdistrict. Betacyanin pigment extraction using maceration method with a ratio of solvent : the sample (1: 1), then the separation using a centrifuge and filtered, the filtrate obtained was concentrated using a rotary evaporator at 35 C to obtain a concentrated extract of 4 ml. The extraction is performed three times. The extract was tested qualitatively using reagent HCl 2 M and NaOH 2 M and for quantitative assay using UV-Vis spectrophotometry at wavelength of 538 nm. The results obtained in qualitative tests showed the three sampling sites containing betacyanin. On the quantitative test results betacyanin total levels from Mantikulore subdistrict of 0.022 mg/100 g, East Palu subdistrict of 0.021 mg/100 g and South Palu subdistrict of 0.021 mg /100 g.

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Published

2016-08-30

Issue

Section

Research Articles

How to Cite

1.
Study of betacyanin level from cactus fruit (Opuntia elatior Mill) in Palu city using spectrophotometry UV-VIS. Int J of Phytopharm [Internet]. 2016 Aug. 30 [cited 2025 Mar. 14];6(4):85-9. Available from: https://ssjournals.co.in/index.php/ijpp/article/view/3085