Biosynthesis of gold nanoparticles (AuNPs) from C. orchioides and study their antimicrobial efficacy

Abstract

In this study, silver nanoparticles were synthesized using the C. orchioides plant extract as a reducing agent. The bioactive phytochemicals/ secondary metabolites present in the plant and were responsible for the quick reduction of silver ion (Au ⁺ ) to metallic silver nanoparticles (Au ⁰ ). The reduced silver nanoparticles were characterized by UVvis spectroscopy, Scanning electron microscopy (SEM), Fourier Transform-Infra Red Spectroscopy (FT-IR), Dynamic light scattering (DLS) and Zeta potential analysis. The UV-VIS spectroscopic studies revealed the presence of beard peaks at around 540 nm. SEM studies showed spherical-shaped gold nanoparticles at 90 nm in higher densities. The microbial derived gold nanoparticles were also showing more inhibition activity in both bacterial and fungus strains. In bacteria, gram negative strains are highly affected by the test samples than gram positive. In fungal strains, the highest effect was noticed in Aspergillus niger while less effect was observed in Candida albicans . The plant materials mediated synthesis of silver nanoparticles have comparatively rapid and less expensive and wide application to antibacterial therapy in modern medicine.