Phytochemical screening and studies of analgesic potential of Moringa oleifera Lam. stem bark extract on experimental animal model

Abstract

The work has been done for the phytochemical investigation and study of analgesic activity of Moringa oleifera Lam. ethanolic stem bark extract using Acetic Acid Induced Writhing method. The effect of extract was tested for qualitative chemical analysis which reveals the presence of alkaloid, glycosides, flavonoids, tannins, saponin, carbohydrate etc. For peripheral analgesic effect acetic acid induced writhing test was used and for this stem bark extract was administered intraperitoneally at doses of 250 mg/kg and 500 mg/kg of body weight to young Swiss-albino mice. Both doses of the extract significantly inhibited writhing response induced by acetic acid in a dose dependent manner which is comparable to the positive control drug Diclofenac Na. These two different doses were found to exhibit 13.22% and 28.94% writhing inhibitory response respectively where the Diclofenac Na inhibited about 42.15% of writhes at a dose of 100mg/kg of body weight. The obtained results provide promising baseline information for the potential use of these crude extract in the treatment of pain.