Screening of BADH Activity of Borreria articularies (Linn.) for the Inhibition of P. aeruginosa

Abstract

Purposes: The present study was designed to investigate the antibacterial activities of the Ethanol and methanol extracts of the leaves of the plant Borreria articularies (Linn.) effects on microbial growth inhibition in vitro, microbial cells in vivo and molecular enzyme (BADH) targets in vitro. Methods: The preliminary phytochemicals of the extracts was determined by the standard methods and aliquoted with Thin Layer Chromatography (TLC) and stored at 2-4^oC. fluorescein diacetate (FDA) and ethidium bromide (EB) live-dead cell viability test for distinguishing the membrane active phytochemicals of the plant extract. Betaine aldehyde dehydrogenase (BADH) activity was assessed by spectrophotometer. Alkaloids, glycosides, steroids, gums, saponin and reducing sugar were found in extracts. Results: The results of the disc diffusion indicated that the crude extracts were able to inhibit the growth of bacteria within a concentration range of 0.5 to 2.0 mg/mL. At a similar concentration range (0.5 to 2.0 mg/mL) the extract inhibited the growth of 90.12% of the tested microorganisms. Bacterial cell viability was found minor in the phytochemicals of crude extract. Also, constituents of crude extract inhibited the BADH activity to protect the adaptation in stress environment of the bacteria. Conclusion: Results of the present study showed the possible use of the studied plants extracts in the control of bacterial infections.