Development of validated HPTLC method for the estimation of betasitosterol in marketed herbal fromulation of muscle and joint HRX pain relieving oil

Authors

  • Manoj S. Charde Government College of Pharmacy, Kathora Naka, Amrawati-444604 Maharashtra
  • M. D. Chakolkar Government College of Pharmacy, Kathora Naka, Amrawati-444604 Maharashtra
  • A. S. Welankiwar Government College of Pharmacy, Kathora Naka, Amrawati-444604 Maharashtra
  • U. Keshwar Baidyanath Life Sciences Pvt. Ltd Nagpur
  • B. K. Shrikande Baidyanath Life Sciences Pvt. Ltd Nagpur

DOI:

https://doi.org/10.7439/ijpp.v4i2.93

Keywords:

socioeconomic status, Diabetes Mellitus, Perimenopausal Women

Abstract

A new and simple HPTLC method was developed and validated for the quantitative estimation of betasitosterol in muscle and joint pain relaxant herbal oil. TLC aluminium plates precoated with silica gel 60F-254 (0.2 mm thickness) were used. The linear ascending development was carried out in twin trough glass chamber saturated with mobile phase Tolune: Ethyl acetate (7:3) ratio followed by densitometric determination was carried out by TLC scanner (CAMAG) at 560 nm in reflectance/absorbance mode. The Rf value was found to be 0.58. Linearity was found to be in the concentration range of 500 ng to 3000 ng. The linear regression data for the calibration plots showed a good linear relationship with r2=0.99 for Betasitosterol.According to the ICH guideline the method was validate for accuracy, precision, Specificity and ruggedness.The proposed method is accurate, precise, reproducible, and can be adopted for routine analysis of Betasitosterolfrom herbal joint and muscle pain relieving oil by HPTLC.

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Author Biography

  • Manoj S. Charde, Government College of Pharmacy, Kathora Naka, Amrawati-444604 Maharashtra
    Assitant Professor

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Published

2014-04-30

Issue

Vol. 4 No. 2 (2014): Mar-Apr

Section

Research Articles

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How to Cite

1.
Development of validated HPTLC method for the estimation of betasitosterol in marketed herbal fromulation of muscle and joint HRX pain relieving oil. Int J of Phytopharm [Internet]. 2014 Apr. 30 [cited 2025 Mar. 14];4(2):70-4. Available from: https://ssjournals.co.in/index.php/ijpp/article/view/1183