Bioautography screening of Jatropha gossypsifolia L. Leaves against the Gram positive organisms

Abstract

Jatropha gossypifolia L ., belonging to the family Euphorbiaceae is traditionally used in curing various ailments. It is commonly called as cotton leaf, physic nut and Belly ache. In Tamil commonly called as Kattamanakku. Humans are natural hosts for many bacterial species that colonize the skin as normal flora. Staphylococcus aureus and Streptococcus epidermis is infrequent resident flora, but they account for a wide variety of bacterial pyodermas. Predisposing factors to infection include minor trauma, pre-existing skin disease, poor hygiene, and rarely, impaired host immunity. The present study deals with the antibacterial activity and phytochemical screening of Jatropha gossypifolia . Sequential extraction was carried out using solvents such as chloroform, ethanol, and water from leaf of the plant were investigated for preliminary phytochemical and antibacterial property. Results of the study showed that all the extracts had good inhibitory activity against Gram-positive test organism. Although all four extracts showed promising antibacterial activity against test bacterial species, yet maximum activity was observed in ethanol extract. The minimum inhibitory concentration ranged in between 64 to 1024 ? g/ml. Evaluation of phytochemicals such as alkaloids, flavonoids, saponin, tannin, glycosides, phenolic compound, proteins and amino acids revealed the presence of most of constituents in polar extracts of aqueous. All the findings exhibit that the leaf extracts have broad-spectrum activity and suggest its possible use in treatment of infectious diseases.