Anti-oxidant activity of methanolic extract of Ficus religiosa linn bark by using DPPH ((1, 1-Diphenyl-2-Picrylhydrazyl)

Abstract

Ficus religiosa is also called as Peepal belongs to family Moraceae, is potentially distributed in india-sub Himalaya,bihar, Orissa, and west Bengal. The tree is found wild or cultivated nearly thought India is considered to be one of the world's most invasive tree and has the potential to spread across central India and upper genetic plain.The medicinal values of plant lie in their component phytochemical such as sterols, terpenoid, coumarins, tannins, and other nutrients like as amino acid, proteins, which produce a definite physiological action on the human body. The present study is aimed an Antioxidant activity of methanolic extracts by using DPPH (1,1-diphenyl-2-picrylhydrazyl) DPPH assay is based on the measurement of the scavenging ability of antioxidant towards the stable DPPH radical. DPPH is a stable free radical at room temperature and accepts an electron or hydrogen radical to become a stable diamagnetic molecule.The reduction capability of the DPPH radical is determined by the decrease in its absorbance at 517 nm, induced by antioxidants.The absorption maximum of a stable DPPH radical in methanol was at 517 nm.