Development of validated HPTLC method for the estimation of eugenol in marketed herbal formulation of muscle and joint HRX pain relieving oil

Authors

  • Manoj S. Charde Government College of Pharmacy, Kathora Naka, Amrawati-444604 Maharashtra
  • M. D. Chakolkar Government College of Pharmacy, Kathora Naka, Amrawati-444604 Maharashtra
  • A. S. Welankiwar Government College of Pharmacy, Kathora Naka, Amrawati-444604 Maharashtra
  • U. Keshwar Baidyanath Life Sciences Pvt. Ltd Nagpur
  • B. K. Shrikande Baidyanath Life Sciences Pvt. Ltd Nagpur

DOI:

https://doi.org/10.7439/ijpp.v4i1.90

Keywords:

SGD- Structured Group Discussion, PBL- Problem Based Learning

Abstract

A new and simple HPTLC method was developed and validated for the quantitative estimation of Eugenol in muscle and joint pain relaxant herbal oil. TLC aluminium plates precoated with silica gel 60F-254 (0.2 mm thickness) were used. The linear ascending development was carried out in twin trough glass chamber saturated with mobile phase Tolune: Ethyl acetate (9.3:0.7) ratio followed by densitometric determination was carried out by TLC scanner (CAMAG) at 560 nm in reflectance/absorbance mode. The Rf value was found to be 0.58. Linearity was found to be in the concentration range of 24 ng to 64 ng. The linear regression data for the calibration plots showed a good linear relationship with r2=0.99 for Eugenol. According to the ICH guideline the method was validate for accuracy, precision, Specificity and ruggedness. The proposed method is accurate, precise, reproducible, and can be adopted for routine analysis of eugenol from herbal joint and muscle pain relieving oil by HPTLC.

Downloads

Download data is not yet available.

Author Biography

  • Manoj S. Charde, Government College of Pharmacy, Kathora Naka, Amrawati-444604 Maharashtra
    Assitant Professor

Downloads

Published

2014-02-27

Issue

Vol. 4 No. 1 (2014): Jan-Feb

Section

Research Articles

License

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).

How to Cite

1.
Development of validated HPTLC method for the estimation of eugenol in marketed herbal formulation of muscle and joint HRX pain relieving oil. Int J of Phytopharm [Internet]. 2014 Feb. 27 [cited 2025 Mar. 14];4(1):28-32. Available from: https://ssjournals.co.in/index.php/ijpp/article/view/1174