Preliminary hepatoprotective activity of Jigarine Cl on carbon tetra chloride induced hepatic damage in rats

Abstract

Jigarine CL is an herbal preparation of Hamdard laboratories waqf Pakistan that has been used as hepatoprotective agent. In this study, the protective effects of Jigarine CL against liver damage were evaluated in carbon tetrachloride (CCl ₄ )-induced chronic hepatotoxicity in rats. Sprague-Dawley (SD) rats were divided in to four groups (I, II, III, IV). Groups I received olive oil 10ml/kg p.o served as control and group II and III were orally fed with Jigarine CL (20ml/kg) for 14 consecutive days while Groups IV were orally fed with saline. After the last dose of jigarine CL and saline group III and IV received a single dose of CCl ₄ (0.3 ml/kg body weight in a 20% olive oil) was injected intraperitoneally 30 min after the last dose of Barrisal and the animals were starved for 24 h. The degree of hepatic-protection was measured using biochemical parameters (serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) and Bilirubin) and histopathology. Data obtained from results were compared using students t-test. The results showed that the treatment of Jigarine CL significantly lowered the CCl ₄ -induced serum levels of hepatic enzyme markers (GOT, GPT and total bilirubin) indicated hepatoprotective effect of the Jigarine CL. Histopathological examination of liver sections confirmed that, pre-treatment with Jigarine CL decreased the degree of hepatic damage induced by CCl ₄ . The significant reversal of the biochemical and histological changes induced by CCl ₄ treatment in rats, indicating promising hepatoprotective activity of Jigarine CL in rats.