LC-MS Quantification of Mangiferin in hydroalcoholic extract of Salacia oblonga, Salacia roxburghii and polyherbal formulation

Abstract

Mangiferin is the one of the major active constituents in the tubers of the Salacia species, which is one of the valuable species in Ayurveda with antidiabetic, antihypertensive, hepatoprotective, anticaries and anticancer potentials. A simple and rapid liquid chromatography coupled mass spectrometry analysis was developed for the quantification of Mangiferin in two species of Salacia and also in polyherbal formulation. This method allowed the direct coupling of an electron spray mass selective detector for the LC system with photo diode array detector, Phenomenex C₁₈ column, acetonitrile and aqueous formic acid (0.5%) as mobile phase. Under these conditions, Mangiferin was well separated from the mixture of components present in the extract and detected with mass spectrometry (mass selective detector).Liquid chromatography coupled with electron spray mass was used in positive ion mode and detected at m/z 423(M+1)⁺. The proposed method is more accurate and sensitive can be used for the routine quantification of the Mangiferin in the herbal extracts as well as polyherbal formulations.