The study of Analgesic, Antidiarrhoeal and Anti-oxidant Effect of Ethanolic Extracts of Ecbolium linnaenum in Albino Mice

Abstract

The Ecbolium linnaenum(leaves) is used as a folk medicine in Bangladesh for pain, diarrhea and infectious diseases. Phytochemical evaluation of the ethanolic extracts of Ecboliumlinnaenumleaves demonstratesthese pharmacologic effect for the presence of alkaloids, tannins, gums,flavonoids and absence of carbohydrates, steroids, saponins. In this present study an attempt was made to determine the analgesic, antidiarrhoel, antioxidantand antimicrobial effectin Swiss Albino mice. Ethanolic extracts of250 and 500 mg/kg showed significant inhibition of writhing reflex 36.20% (P 0.01) and 54.48% (P 0.001), respectively while the standard drug diclofenac-Na was 75.52% (P 0.001) at a dose of 25 mg/kg body weight.In the castor oil-induced diarrhoealmice, the ethanolic extracts of 250 mg/kg and 500 mg/kg, raised the latent period and reduced the number of stools comparing with standard drug Loperamide. 0.02% DPPH solution of ethanol on TLC plate showed the presence of anti-oxidant components in the Ecboliumlinnaenum.From the % inhibition of ascorbic acid and Ecboliumlinnaenum we observe that it has anti-oxidation effect. The IC50 (inhibitory conc. 50%) for ascorbic acid is approximately 1 g/ml and for the sample it is more than 500 g/ml. The ethanolic extract of Ecboliumlinnaenum was tested for antimicrobial activity against a number of both gram positive and gram-negative bacteria but it does not show any anti-microbial effect.