IMPROVED RP-HPLC METHOD FOR QUANTITATIVE ESTIMATION OF STEVIOSIDE IN STEVIA REBAUDIANA BERTONI BURM

Authors

  • Shankar Katekhaye Medicinal Natural Products Research Laboratory, Pharmaceutical Division, Institute of Chemical Technology (ICT), Nathalal Parikh Marg, Matunga (E), Mumbai, India 400 019.
  • D. B. Jadhao Advanced Drying Laboratory, Department of Chemical Engineering, Institute of Chemical Technology (Formerly UDCT), N. P. Road, Matunga (E), Mumbai 400 019, India.
  • B. N. Thorat Advanced Drying Laboratory, Department of Chemical Engineering, Institute of Chemical Technology (Formerly UDCT), N. P. Road, Matunga (E), Mumbai 400 019, India.

DOI:

https://doi.org/10.7439/ijpp.v1i2.162

Abstract

An RP-HPLC method with UV array detection was established for the determination of stevioside, an extract of herbal S. rebaudiana plant . The stevioside was separated using isocratic solvent system consisting of methanol and 0.1% orthophosphoric acid (v/v) in water (70:30) at flow rate of 1.0 ml/min and the detection wavelength of 219 nm. The method was validated for linearity, precision, accuracy, limit of detection (LOD), and limit of quantitation (LOQ). The linearity of the proposed method was obtained in the range of 5.0-75 μg/ml with regression coefficient of 0.9999. Intraday and interday precision studies showed the relative standard deviation less than 2.5%. The accuracy of the proposed method was determined by a recovery study conducted at 3 different levels. The average recovery was 97-99%. The LOD and LOQ were 0.02 and 0.05

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Published

2011-01-02

Issue

Vol. 1 No. 2 (2011): Nov- Dec

Section

Research Articles

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How to Cite

1.
IMPROVED RP-HPLC METHOD FOR QUANTITATIVE ESTIMATION OF STEVIOSIDE IN STEVIA REBAUDIANA BERTONI BURM. Int J of Phytopharm [Internet]. 2011 Jan. 2 [cited 2025 Mar. 14];1(2):pp. 27-34. Available from: https://ssjournals.co.in/index.php/ijpp/article/view/1124