ISOLATION AND HPLC METHOD DEVELOPMENT FOR FILIXIC ACID PBP FROM DRYOPTERIS FILIX-MAS

Authors

  • Shankar Katekhaye Medicinal Natural Products Research Laboratory, Pharmaceutical Division, Institute of Chemical Technology (ICT), Nathalal Parikh Marg, Matunga (E), Mumbai, India 400 019.
  • P. B. Shinde Medicinal Natural Products Research Laboratory, Pharmaceutical Division, Institute of Chemical Technology (ICT), Nathalal Parikh Marg, Matunga (E), Mumbai, India 400 019.
  • K. S. Laddha Medicinal Natural Products Research Laboratory, Pharmaceutical Division, Institute of Chemical Technology (ICT), Nathalal Parikh Marg, Matunga (E), Mumbai, India 400 019.

DOI:

https://doi.org/10.7439/ijpp.v1i1.156

Abstract

Simple, rapid and specific isolation and HPLC method has been develop for filixic acid PBP in Dryopteris filix-mas extract. HPLC analysis was performed on C18 column using a 90:10 ( v / v ) mixtures of acetonitrile and methanol as isocratic mobile phase at a flow rate 1.0 ml/min. UV detection was at 254 nm for filixic acid PBP. Filixic acid shown retention time at 4.02 min. Method was validated for accuracy, precision, linearity, specificity and sensitivity in accordance with International Conference on Harmonisation guidelines. Validation studies revealed that the method is specific, accurate, precise, reliable and reproducible. Good linear correlation coefficient (r2 > 0.993) was obtained for calibration plots in the range tested. The limit of detection was 2.25

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Published

2011-11-01

Issue

Vol. 1 No. 1 (2011): Sep-Oct

Section

Research Articles

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How to Cite

1.
ISOLATION AND HPLC METHOD DEVELOPMENT FOR FILIXIC ACID PBP FROM DRYOPTERIS FILIX-MAS. Int J of Phytopharm [Internet]. 2011 Nov. 1 [cited 2025 Mar. 14];1(1):pp. 01-07. Available from: https://ssjournals.co.in/index.php/ijpp/article/view/1117