Histomorphometric study for volume proportion of certain parameters in placenta of pregnancy induced hypertension

Abstract

Introduction: placenta is vital link between mother and fetus. The organ for pregnancy serves as exchange membrane and hormone synthesizing apparatus for both mother and fetus. All physiological processes occurring in fetus or mother are reflected on placenta. Pregnancy induced hypertension is such a co-morbid condition which affects mother and fetus as well as placenta. Aim and objective: To study the desired histomorphometrical parameters in control and experimental (PIH) group of placentae after staining with Hematoxylin and Eosin(H/E) and Masson trichrome to find out the statistical relevance of the comparative findings. Material and method: Forty (40) control and forty (40) experimental placentae (PIH) were collected and processed for routine histological techniques. Paraffin sections were stained with routine H/E and Masson trichrome stain. Light microscopy for histological and histomorphometrical study was performed to obtain volume proportion of fibrinoid degeneration and microscopic calcification. Observation: volume proportion of fibrinoid degeneration and microscopic calcification were significantly increased in PIH cases in comparison to control.