Pharmacological Evaluation and Phytochemical Screening of Dactylorhiza Hatagirea on Anti –Ulcer Activity

Abstract

In the present work, the acute toxicity carried out based on OECD-423 rules for chloroform extract of Dactylorhiza hatagirea prove that, the doses of 250 and 400 mg/kg did not indication any sign of toxicity and mortality. Hence these doses of the concentrate were chosen for assessment of anti-ulcer activity. Dactylorhiza hatagirea in the highest dose tested (400 mg/kg), shows increased in Gastric pH and Gastric juice pH, whereas decrease in Gastric content, Gastric juice volume and Total acidity. Therefore, as per histopathological evaluation studies, it was concluded that, Dactylorhiza hatagirea, at the highest dose of 400 mg/kg, found to be safe and more effective in eradicating gastric ulceration. In Conclusion, based on the results obtained the chloroform extract of Dactylorhiza hatagirea treated groups demonstrates a critical impact when contrasted with control group animals which showing that the plant having the having the anti- ulcer activity. The antiulcer action of Dactylorhiza hatagirea was assessed by pylorus ligation instigated ulcer models. These models cause the gastric ulcer in people. Numerous variables and instruments are associated with the ulcerogensis and gastric mucosal harm. Pylorus ligation induced ulcer was utilized to note the impact of Dactylorhiza hatagirea extract on gastric acid secretion and bodily fluid emission. The ligature of the opening finish of the abdomen causes accumulation of internal organ acid within the abdomen. This increase within the internal organ acid secretion causes ulcers within the abdomen. Ligation of pyloric end of the stomach is made in 24 h fasted rats; the UI is resolved 4 h after pylorus ligation. The lesions created by this methodology are placed inside the lumen area of abdomen.