Study of oxidative stress and endogenous antioxidant defense in patients with beta thalassemia major
Keywords:
Beta-thalassemia major, Oxidative stress, Endogenous Antioxidants, Malondialdehyde, Ferritin, CeruloplasminAbstract
Background: Beta-thalassemia major is an autosomal recessive disease causing severe and hemolytic anemia, which begins about 2-6 months after birth. Iron overload, which arises from recurrent transfusion and ineffective erythropoiesis, can enhance oxidative stress in thalassemic patients. The aim of present study was to assess the oxidative stress and endogenous antioxidants in beta thalassemia major patients.
Method: The study comprises of 49 diagnosed beta thalassemia major patients receiving regular blood transfusion and chelation treatment and 49 age and sex matched normal healthy controls. 5mL blood was collected from patients and controls in plain bulb. The serum was separated and analyzed as per procedure of biochemical parameters such as Iron, ferritin, uric acid, ceruloplasmin, bilirubin and serum malondialdehyde (MDA) in patients and compared with normal healthy controls.
Results: The MDA level increased in β thalassemia major patients (3.01±0.38) as compared to controls (1.68±0.2). Serum free iron, serum ferritin and ceruloplasmin concentration as well as total bilirubin level was high in study group. Uric Acid, an endogenous antioxidant was significantly increased while calculated value of transferrin was decreased in cases than controls.
Conclusion: We conclude that in patients with β thalassemia, the body generates and promotes endogenous antioxidants to defend the oxidative stress. For beneficial effects along with iron chelators, dietary supplementation of antioxidants should be given.
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