Evaluation of Anticancer activity of Acorus calamus using in silico and in vitro models.
Anticancer activity of saponin isolated from Acorus calamus
Keywords:
Acorus calamus, Human breast cancer MCF -7 cells, MTT assay, Angiogenesis assay, Chromosomal aberration assay, Apopsis assay.Abstract
The present study evaluated the anticancer action of saponin rich fraction of Acorus calamus by the use of in silico and in-vitro models. In silico studies of 1α, 2β, 3γ, 19α-tetrahydroxyurs-12en-28-oicacid-28-O{-β-Dglucopyranosyl (1→2)} β- D- galactopyranoside and 3β, 22α, 24,29-tetrahydroxyolean-12-en-3-O-{-β-D-arabinosyl(1→3)}-β-D-arabinopyranoside was performed via PASS Online and SwissTargetPrediction softwarae for the prediction of structure based pharmacological activities and docking studies with Autodock Vina. Saponin rich fraction was examined for its effects on growth of MCF-7 cells using MTT antiproliferative assay. Angiogenic property was assessed by in vitro shell less cultures of chick embryo using different (3µg/ml, 6µg/ml, and 12µg/ml) concentrations. Chromosomal aberration assay was studied in vitro in cultured human blood after the treatment of saponin rich fraction for the physical reliability of chromosomes. Apoptogenic prospective of saponin rich fraction was also assessed in MCF-7 cells by using cleaved caspases-3 and cleaved caspase-8. MTT assay result demonstrated IC50 value of saponin rich fraction at 6μg/mL in MCF-7 cells. Angiogenic parameters showed significant (p<0.05) decline after the treatment. Insignificant chromosomal aberrations were observed in normal blood cells. Treatment of saponin rich fraction stimulates caspases-3 (OD 0.04 at 450 nm) and caspase-8 (OD 0.08 at 450 nm) in MCF-7 cells.
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