Effect of tamoxifen on ethanol induced gastric ulcer in rats

Authors

  • Malak Eljafari Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, University of Tripoli. Tripoli, Libya. https://orcid.org/0000-0002-5361-6293
  • al-attar Department of Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.

Keywords:

Tamoxifen, ulcer, anti-oxidants, cytoprotective

Abstract

Background: Tamoxifen is selective estrogen receptor modulators, used in treatment of breast cancer; some literatures reported its impact on the process of peroxidation.

Aim: This study was aimed to evaluate the effect of tamoxifen on ethanol induced gastric ulcer in rats.

Method: Gastric ulcers were induced in Wistar albino rats by oral administration of absolute alcohol (1 ml/200 gm). Antiulcer activity of tamoxifen (0.5 and 10 mg/kg, p.o.) was observed and compared to standard drug (omeprazole 20mg/kg, p.o.), the ulcer index, ulcers numbers, lengths, gastric volume and total gastric acidity were evaluated. Histopathology is performed for confirmation.

Results: tamoxifen in 10mg/kg dose produced a highly significant (P<0.001) decrease in ulcer parameters studied compared to ulcer control. While 0.5mg/kg of tamoxifen produced less significant (P<0.01) effect on gastric volume when compared to ulcer group. The anti-ulcer effects of the drug of interest is highly comparable to omeprazole was very, surprisingly; the higher dose of tamoxifen produced even a much significant reduction in gastric volume and ulcers length compared to standard drug.

Conclusion: Tamoxifen shows significant antiulcer activity against ethanol induced gastric ulcers, and this could possibly related to its antioxidant properties.

Downloads

Download data is not yet available.

References

- Chang M. Tamoxifen resistance in breast cancer. Biomol Ther (Seoul) 2012 May; 20(3):256-67. Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794521/

- Martinkovich S, Shah D, Planey SL, Arnott JA. Selective estrogen receptor modulators: tissue specificity and clinical utility. Clin Interv Aging 2014 Aug 28;9:1437-52. Available at: https://pubmed.ncbi.nlm.nih.gov/25210448/

- Thangaraju M, Vijayalakshmi T, Sachdanandam P. Effect of tamoxifen on lipid peroxide and antioxidative system in postmenopausal women with breast cancer. Cancer. 1994 Jul 1;74(1):78-82 available at: https://pubmed.ncbi.nlm.nih.gov/8004586/

- Hernández-Esquivel L, Zazueta C, Buelna-Chontal M, Hernández-Reséndiz S, Pavón N, Chávez E. Protective behavior of tamoxifen against Hg2+-induced toxicity on kidney mitochondria: in vitro and in vivo experiments. J Steroid Biochem Mol Biol 2011;127(3-5):345-50. Available at: https://europepmc.org/article/med/21821123

- Belenichev, I.F., Odnokoz, O.V., Pavlov, S.V. et al. The neuroprotective activity of tamoxifen and tibolone during glutathione depletion in vitro. Neurochem. J. 2012;(6) 202–212. Available at: https://doi.org/10.1134/S181971241203004X

- Arab H.H., Salama S.A., Omar H.A., Arafa E.-S.A., Maghrabi I.A. Diosmin Protects against Ethanol-Induced Gastric Injury in Rats: Novel Anti-Ulcer Actions. PLoS ONE. 2015;10:E0122417. Available at: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0122417

- Sistani Karampour N, Arzi A, Rezaie A, Pashmforoosh M, Kordi F. Gastroprotective Effect of Zingerone on Ethanol-Induced Gastric Ulcers in Rats. Medicina (Kaunas). 2019 Mar 11;55(3):64. Available at: https://pubmed.ncbi.nlm.nih.gov/30862060/

- Atakisi E, Kart A, Atakisi O, Topcu B. Acute tamoxifen treatment increases nitric oxide level but not total antioxidant capacity and adenosine deaminase activity in the plasma of rabbits. Eur Rev Med Pharmacol Sci. 2009;13(4):239-243. Available at: https://www.europeanreview.org/article/645

- Tayeby F, Salman AAA, Kamran S, Khaing SL, Salehen NB, Mohan GMAD. Ulcer Prevention Effect Of 3,4,5-Tihydroxy-N0-[(2-Methyl-1H-Indol-3yl)Methylidene]Benzohydrazide In HCl/Ethanol-Induced Gastric Mucosal Damage In Rats. Int J Med Sci. 2017 Oct 15;14(13):1317-1326. Available at: https://pubmed.ncbi.nlm.nih.gov/29200945/

- Süleyman H, Akçay F, Altinkaynak K. The effect of nimesulide on the indomethacin- and ethanol-induced gastric ulcer in rats. Pharmacol Res. 2002 Feb;45(2):155-8. Available at: https://pubmed.ncbi.nlm.nih.gov/11846629/

- Gregory M, Divya B, Mary RA, Viji MM, Kalaichelvan VK, Palanivel V. Anti-ulcer activity of Ficus religiosa leaf ethanolic extract. Asian Pac J Trop Biomed. 2013 Jul;3(7):554-6. Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3695581/

- Ezgallaey HM, Abuskhuna SM, Dugani AM. Gastroprotective effects of 1-Hydroxy-2- phenylbenzimidazole in ethanol-induced gastric ulcers in rats. LIMUJ.2016; (1) 82-92. Available at: https://journal.limu.edu.ly/article.asp?issn=2519-139X;year=2016;volume=1;issue=2;spage=82;epage=92;aulast=Ezgallaey;type=0

- Peskar BM, Ehrlich K, Peskar BA. Role of ATP-sensitive potassium channels in prostaglandin-mediated gastroprotection in the rat. J Pharmacol Exp Ther. 2002 Jun;301(3):969-74. Available at: https://pubmed.ncbi.nlm.nih.gov/12023526/

- Abdallah, I.Z.A., Khattaba, H.A.H. and Heeba G.H. (2011). Gastroprotective Effect of Cordia Myxa L. Fruit Extract against Indomethacin-Induced Gastric Ulceration in Rats. Life Sci. J.; 8(3). 433-445. Available at: http://www.lifesciencesite.com/lsj/life0803/068_6818life0803_433_445.pdf

- Anoop A, Jegadeesan M. Biochemical studies on the anti-ulcerogenic potential of Hemidesmus indicus R.Br. var. indicus. J Ethnopharmacol. 2003 Feb;84(2-3):149-56. Available at: https://pubmed.ncbi.nlm.nih.gov/12648808/

- M. A. Abdulla, K. A. A. Ahmed, F. H. Al-Bayaty, and Y. Masood, “Gastroprotective effect of Phyllanthus niruri leaf extract against ethanol-induced gastric mucosal injury in rats,” African Journal of Pharmacy and Pharmacology, vol. 4, no. 5, pp. 226–230, 2010. Available at: https://academicjournals.org/journal/AJPP/article-full-text-pdf/AB378CA25564/

- El-Maraghy SA, Rizk SM, Shahin NN. Gastroprotective effect of crocin in ethanol-induced gastric injury in rats. Chem Biol Interact. 2015 Mar 5;229:26-35. Available at: https://pubmed.ncbi.nlm.nih.gov/25637687/

- Lanas A. Role of nitric oxide in the gastrointestinal tract. Arthritis Res Ther. 2008;10 Suppl 2(Suppl 2):S4. Available at:https://pubmed.ncbi.nlm.nih.gov/19007429/

- Wiseman H, Laughton MJ, Arnstein HRV, Cannon M and Halliwell B. The antioxidant action of tamoxifen and its metabolites April 1990 Inhibition of lipid peroxidation. FEBS 1990 April;(263):number 2, 192-194. Available at: https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/0014-5793%2890%2981371-T

- Custódio JB, Dinis TC, Almeida LM, Madeira VM. Tamoxifen and hydroxytamoxifen as intramembraneous inhibitors of lipid peroxidation. Evidence for peroxyl radical scavenging activity. Biochem Pharmacol. 1994;47(11):1989-1998. Available at: https://pubmed.ncbi.nlm.nih.gov/8010983/

- Moreira PI, Custodio JB, Oliveira CR, Santos MS. Brain mitochondrial injury induced by oxidative stress-related events is prevented by tamoxifen. Neuropharmacology 2005;(48) 435–447. Available at: https://core.ac.uk/download/pdf/144009009.pdf

- Ek RO, Yildiz Y, Cecen S, Yenisey C, Kavak T. Effects of tamoxifen on myocardial ischemia-reperfusion injury model in ovariectomized rats. Mol Cell Biochem. 2008 Jan;308(1-2):227-35. Available at: https://pubmed.ncbi.nlm.nih.gov/17978864/

- Shin JM, Sachs G. Pharmacology of proton pump inhibitors. Curr Gastroenterol Rep. 2008 Dec;10(6):528-34. Available at: https://pubmed.ncbi.nlm.nih.gov/19006606/

- Huh WJ, Khurana SS, Geahlen JH, Kohli K, Waller RA, Mills JC. Tamoxifen induces rapid, reversible atrophy, and metaplasia in mouse stomach. Gastroenterology. 2012 Jan;142(1):21-24.e7.available at: https://pubmed.ncbi.nlm.nih.gov/22001866/.

Downloads

Published

2021-02-28

Issue

Section

Research Articles

How to Cite

1.
Eljafari M, R. Al-Attar A-A. Effect of tamoxifen on ethanol induced gastric ulcer in rats. Int J of Pharmc Res [Internet]. 2021 Feb. 28 [cited 2025 Mar. 14];11(2):e5555. Available from: https://ssjournals.co.in/index.php/ijpr/article/view/5555