Role of circulating miRNA-9 as a diagnostic marker in a population with diabetic complications

Authors

  • Seema Singh Department of Physiology, Era’s Lucknow Medical College and Hospital, Lucknow
  • Nitin Ranjan Gupta Associate Professor Dept. of Internal Medicine, Hind Institute of Medical Sciences and Research. Ataria, Mau, Sitapur https://orcid.org/0000-0003-2763-0187
  • Syed tahseen raza Department of Physiology, Era’s Lucknow Medical College and Hospital, Lucknow https://orcid.org/0000-0003-3807-2803
  • Rahul Singh Consultant Psychiatrist at District Hospital Barabanki (U.P) https://orcid.org/0000-0002-0592-2499
  • Amresh Bahadur Singh Deputy General Manager SPMU, NHM

Keywords:

Diabetes, blood miRNAs, miR-9, biomarkers

Abstract

Introduction: Diabetes mellitus is an autoimmune disorder which is caused by deregulation of blood glucose homeostatis accompanied by various other disturbances of carbohydrates, fat and protein metabolism. It also involves various other factors such as genetic, epigenetic and environmental factors. The deregulated metabolism of this disease is the failure of β cells to produce compensatory insulin which is a necessary step towards increased insulin resistance.

Material and methods: The present study was conducted over 50 diabetic patients and 50 non-diabetic healthy individuals. Briefly, reverse transcription was carried for cDNA synthesis using Applied Biosystems TaqMan miRNA RT kit (Thermo Fisher Scientific, USA). Statistical analysis used to compare the expression of miRNAs, clinical variables in cases and controls were done by SPSS software.

Results: Our study has revealed the significance of alterations and challenges in peripheral blood miRNA-9 as efficient biomarker for diabetes. The levels of miRNA 9 were found higher in diabetic patients in comparison to control healthy individuals. The mean age difference was found significant. In case of gender no significant difference was found between the two groups. (p<0.05). The glycemic status was found significantly higher in diabetic patients as compared to healthy individuals. No significant difference was found between the two groups in relation to blood pressure, triglycerides, total cholesterol and HDL.

Conclusion: The results revealed that however, a borderline significance and low diagnostic value of miRNA9 was evaluated through results still the fact that miRNA9 expression levels were higher in diabetes patients could not be surpassed and its link with the disease progression could not be foreseen.

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Published

2020-10-30

Issue

Vol. 10 No. 10 (2020): Oct

Section

Research Articles

License

Copyright (c) 2020 International Journal of Pharmacological Research

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How to Cite

1.
Singh S, Gupta NR, raza S tahseen, Singh R, Singh AB. Role of circulating miRNA-9 as a diagnostic marker in a population with diabetic complications. Int J of Pharmc Res [Internet]. 2020 Oct. 30 [cited 2025 Mar. 14];10(10):e5517. Available from: https://ssjournals.co.in/index.php/ijpr/article/view/5517