Toxicological profiles of methanol stem bark extract of Tamarindus indica on experimental hyperglycemic wistar rats
Keywords:
T. indica, hyperglycaemia food and water intake, hematologyAbstract
Introduction: Drinking behavior after water deprivation is one of the standard tests used to study thirst in humans and animals and in addition, diurnal cycle, food availability are known to influence water intake.
Methodology: A diurnal cycle of 24 h water deprivation with free access to food from 1300 h one day to 1300 h the next day, followed by free access to both food and water was used. To determine the level of haematological parameters alloxan was used to induce hyperglycaemia and graded doses of the extract were administered orally for 28 days.
Results: The three doses of the extract used significantly (p 0.05) improved the feeding behaviour of the hyperglycaemic animals after seven days. Water consumption was significantly reduced by all the doses of the extract used in the hyperglycaemic treated animals. The WBC, neutrophils, monocytes, lymphocytes, and eosinophils levels were also analyzed. Administration of graded doses of the extract caused a significant improvement in the level of RBC and its related indices, the 1000 mg/kg dose being the most effective. The 250 mg/kg dose and metformin caused a significant (p 0.05) improvement in the white blood counts and the level of neutrophils, monocyte and lymphocyte were also significantly (p 0.05) restored to near normal values.
Conclusion: The doses of the extract used and metformin also significantly (p 0.05) reduced the water consumption of the animals. Decreases in haematological parameters were restored to near normal levels in the groups treated with the extract.
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