Protective role of Momordica cymbalaria in diethyl nitrosamine induced hepatocellular carcinoma
DOI:
https://doi.org/10.7439/ijpr.v6i1.2895Abstract
Objective: The objective of this study was to evaluate the protective role of Momordica cymbalaria against N-nitroso diethylamine(DEN) induced hepatocelllular carcinoma. Method: The protective effect of the plant extracts were evaluated by inducing Hepatocellular carcinoma in rat by giving single dose of 200mg/kg of N-nitroso diethylamine effect of DEN is potentiated by administering Phenobarbital in water. Various serum biochemical and histo-pathological studies were done to determine the effect of plant extract on hepatocellular carcinoma. The levels of GSH, SOD, LPO and CAT and activities of antioxidant enzymes were assessed in the haemolysate and liver of experimental animals to evaluate the antioxidant status. Result: The results of the biochemical estimations demonstrated that the hepatocellular carcinoma was successfully induced by the DEN whereas the effect of DEN was reversed by the administration of the bark extracts. The antioxidant effect of the plant extracts were proved by estimating various parameters of the liver tissue homogenate. All these results indicate that the methanolic extract of Momordica cymbalaria have chemopreventive effect against DEN induced hepatocellular carcinoma. Conclusion: the present results suggest that saponin of Momordica cymbalaria exerts protective effect by modulating the antioxidant status during DEN induced hepatocarcinogenesis.Downloads
References
Siegel R, Ma J, Zou Z, et al. Cancer statistics, CA cancer J Clin, 2014; 64: 9-29.
Bosch, F.X., Ribes, J. and Borras, J. Epidemiology of primary liver cancer. Shemin. Liver. Dis., 1999; 19: 271-285.
Lindhe, B., Porsch-Hallstrom, I., Gustafsson, J.A. and Blanck, A. Effects of neonatal and adult castration and of testoster- one substitution in male rats on growth of enzyme-altered hepatic foci in the resistant hepatocyte model. Cancer Res., 1990; 50: 2679-2682.
Nakatani, T., Roy, G., Fujimoto, N., Asahara, T. and Ito, A. Sex hormone dependency of diethylnitrosamine-induced liver tumors in mice and chemoprevention by leuprorelin. Jpn. J. Cancer Res., 2001; 92: 249-256.
Kang, J.S., Ahn, B., Kim, C.K., Han, B.S., Che, J.H., Kim, S., Jang, D.D. and Yang, K.H. Suppression of chemically- induced liver tumors by castration or estradiol-3-benzoate treat- ment in F344 rats. Oncol. Rep., 2005; 14: 377-382.
Dragan, V.P., Vaughan, J., Jordan, V.C. and Pitot, H.C. Comparison of the effects of tamoxifen and toremifene on liver and kidney tumor promotion in female rats. Carcinogenesis, 1995; 16: 2733-2741.
Dragan, Y.P., Xu, Y.D. and Pitot, H.C. Tumor promotion as a target for estrogen/antiestrogen effects in rat hepatocarcino- genesis. Prev. Med., 1991; 20: 15-26.
El-Serag, H.B. and Rudolph, K.L. Hepatocellular carci- noma: epidemiology and molecular carcinogenesis. Gastroen- terology, 2007; 132: 2557-2576.
El-Serag HB, Rudolph KL. Hepatocellular carcinoma: epidemiology and molecular carcinogenesis. Gastroenterology 2007; 132:2557
McKillop IH, Schrum LW. Role of alcohol in liver carcinogenesis. Semin Liver Dis 2009; 29:222
Voigt MD. Alcohol in hepatocellular cancer. Clin Liver Dis 2005; 9:151
Brandon-Warner E, Walling TL, Schrum LW, McKillop IH. Chronic ethanol feeding accelerates hepatocellular carcinoma progression in a sexdependent manner in a mouse model of hepatocarcinogenesis. Alcohol Clin Exp Res 2012; 36:641
Veerendra KP. Evaluation of antidiabetic activity of Momordica cymbalaria and Momordica dioica [dissertation]. Visveswarapura institute of pharmacy: Rajiv Gandhi University of Health Sciences, Bangalore 2007.
Raju K, Balaraman R. Antidiabetic mechanism of saponins of Momordica cymbalaria. Phcog Mag 2008; 4(15):197-205.
Raju K, Balaraman R, Hariprasad, Vinoth KM. Cardioprotective effect of Momordica cymbalaria Fenzl in rats with isoproterenol-induced myocardial Injury. Journal of Clinical and Diagnostic Research 2008; 2:699-705.
Elizabeth A. Evaluation of antifertility activity of roots of Momordica cymbalaria Fenzl [dissertation]. Visveswarapura institute of pharmacy: Rajiv Gandhi University of Health Sciences, Bangalore 2005.
Mushirbhai M. Evaluation of antihyperlipidimic activity of saponins fraction of roots of Momordica cymbalaria [dissertation]. Visveswarapura institute of pharmacy: Rajiv Gandhi University of Health Sciences, Bangalore 2007.
Koneri R, Balaraman R, Saraswathi CD. Antiovulatory and abortifacient potential of the ethanolic extract of extract of roots Momordica cymbalaria Fenzl in rats. Indian J Pharmacol. 2006; 38:111-14.
Firdous SM, evaluation of hepatoprotective, analgesic and anti-inflammatory activity of saponins of Momordica cymbalaria Fenzl and Momordica dioica Roxb [dissertation]. Visveswarapura institute of pharmacy: Rajiv Gandhi University of Health Sciences, Bangalore 2007.
Koneri R, Balaraman R, Firdous, Vinoth K M. Hepatoprotective Effects of Momordica cymbalaria Fenzl against carbon tetrachloride Induced hepatic Injury in Rats. Pharmacologyonline. 2008; 1:365-374.
Jahn MS,vani G, shyamaladevi CS. Anti-carcinogenic effect of solanum trilobatum in diethyl nitrosamine induced and phenobarbital promoted hepatocaarcinogenesis in rats. Asian J Biochem 2011; 6(1):74-81.
Surender RP, Hari KL, Ashok RV, Narshima RY. Chemoprotective effect of Bombax malabaricum DC against N-Nitrosodiethylamine induced hepatocellular carcinoma. Int J Curr pharm Res 2011, 3(1):93-6.
Fauziah O, Hanchi P, Yogespiriya S, Asmah R, Reducing effect of strobilanthes crispus leaf in hepatocarcinogenesis Rats. Int J cancer Res 2005; 1(2):109-12.
Natesan S, Badami S, Dongre SH, Godavarthi A, Antitumor activity and antioxidant status of methanol extract of careya arborea bark against Dalton
Xitusan Z, Huilin Z, Shuwei Z, Qiang Y and Lijiang X, Sesquiterpenoids from Bombax malabaricum, J. Nat. prod 2007;70(9):1526-1528.
Urtusan RC Chapman JD, Raleigh JA, Franko AJ, and Koch CJ, Binding of 3H- misonidazole to solid human tumors as a measure of tumor hypoxia, International Journal of Radiation Oncology, Biology, Physics 1986;12:1623-7.
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