Immunomodulation Potential of Andrographis Paniculata and Tinospora Cordifolia Methanolic Extracts in Combination Forms
DOI:
https://doi.org/10.7439/ijpr.v6i1.2822Keywords:
Pregnancy induced hypertension, maternal outcome, perinatal outcomeAbstract
Objective: This work mainly focuses on the immunomodulatory effect of different drug combinations (DC-I to DC-V) of methanolic extracts of aerial parts of Andrographis paniculata (AP) and mature stems of Tinospora cordifolia (TC).
Materials and Methods : The coarse powder (40-mesh) of shade dried aerial parts of AP and mature stems of TC (500g each) was subjected separately tosuccessive extraction with 1000ml each of petroleum ether (60-80oC) followed by methanol. The doses of DC-I to DC-V were selected and were administered orally at doses of 200 mg/kg body weight to Albino Wistar rats and compared with the control group and standard drug i.e. cyclophosphamide (100mg/kg) respectively.
Results: Results suggest that although all the five tested combinations (DC-I to DC-V) prepared using different proportion of methanol extracts of A. paniculata and T. cordifolia (100:Zero, 75:25, 50:50, 25:75 and Zero:100) showed a significant increase in macrophase count, neutrophil adhesion(NA), haemagglutinating antibody (HA) titre and delayed type hypersensitivity (DTH) response. In rats immunized with sheep RBC, DC-I to DC-V enhanced the humoral antibody response to the antigen and significantly potentiated the cellular immunity by facilitating the footpad thickness response to sheep RBC in sensitized rats on chosen experimental models.
Conclusion: The values of macrophase, NA, HA, DTH and cellular responses of combination DC-II at a 200 mg/kg body weight were statistically significant as compared to other combinations. Combination DC-II exhibited best immunostimulant potential, indicating that it has promising immunomodulatory activity, as proposed from commercial point of view.
Downloads
Downloads
Published
Issue
Section
License
Copyright (c) 2016 International Journal of Pharmacological Research
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
- Author must Submit Copyright form After acceptance of Article
How to Cite
