Water extract of Osmanthus Fragrans attenuates TGF-?1-induced lung cellular fibrosis in human lung fibroblasts cells.

Authors

  • Yu-Lin Yang Graduate Institute of Biomedical Science, Chung Hwa University of Medical Technology, Tainan, Taiwan
  • Shu-Jhen Ma Graduate Institute of Medical Laboratory Science and Biotechnology, Chung Hwa University of Medical Technology, Tainan
  • Shu-Fen Liu Department of Internal Medicine, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung
  • Pei-fang Hsieh Graduate Institute of Biomedical Science, National Sun Yat-sen University, Kaohsiung
  • Yu-Lin Yang Graduate Institute of Biomedical Science, Chung Hwa University of Medical Technology, Tainan

DOI:

https://doi.org/10.7439/ijpr.v5i8.1951

Abstract

Introduction: Idiopathic interstitial pneumonia, also known as idiopathic pulmonary fibrosis, is a type of diffuse parenchymal lung disease of unknown etiology. Osmanthus Fragrans, referred to as sweet olives, possesses various functions, including neuro-protection, the scavenging of free radicals, and anti-oxidation. Transforming growth factor beta (TGF-?) is a vital growth factor that is essential for regulating cell growth, differentiation, immune-regulation, and extracellular matrices. This study investigates the mechanism underlying water extract of Osmanthus fragrans on the regulation of pulmonary cellular fibrosis. Materials and Methods: The HFL-1 cells, human lung fibroblasts, were cultured with 5 ng/mL of TGF-?1 for 24 h. After the induction of cellular fibrosis, 200 ?g/mL water extract of Osmanthus fragrans was added and cultured for another 24 h. Subsequently, the enzyme-linked immunosorbent assay was performed to quantify the extracellular fibronectin, and Western blot analysis was conducted to examine the expression of TGF-?1 and its downstream SMAD proteins. Results: The findings indicated that 200 ?g/mL of water extract of Osmanthus fragrans significantly attenuates TGF-?1-induced increase in fibronectin. In addition, Osmanthus fragrans dramatically suppresses TGF-?1-induced increases in the expression of pSmad2/3. Conclusion: We propose that water extract of Osmanthus fragrans is a potential fibrosis antagonist for lung fibroblasts. Thus, water extract of Osmanthus fragrans may exert its beneficial effects through suppressing post-receptor signaling of TGF-?1 and restoring lung fibroblasts cells character by blocking the expression of pSmad2/3.

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Published

2015-09-03

Issue

Vol. 5 No. 8 (2015): Aug

Section

Research Articles

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How to Cite

1.
Yang Y-L, Ma S-J, Liu S-F, Hsieh P- fang, Yang Y-L. Water extract of Osmanthus Fragrans attenuates TGF-?1-induced lung cellular fibrosis in human lung fibroblasts cells. Int J of Pharmc Res [Internet]. 2015 Sep. 3 [cited 2024 Oct. 18];5(8):191-9. Available from: https://ssjournals.co.in/index.php/ijpr/article/view/1951

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