In vitro antioxidant assay of selected aqueous plant extracts and their polyherbal formulation

Authors

  • Ganga Raju M. Associate Professor and Head, Department of pharmacology, Gokaraju Rangaraju of College of Pharmacy, Bachupally, Hyderabad. Telangana State,
  • Satyanarayana S. Principal, Avanthi Institute of Pharmaceutical Sciences, Cherukupally, Vizianagaram district, Andhra Pradesh
  • Eswar Kumar K. Department of Pharmaceutical Sciences, Andhra University, Vishakapatnam, Andhra Pradesh

DOI:

https://doi.org/10.7439/ijpr.v5i4.1927

Keywords:

Amalgam, low copper, Uses, Advantages, patents

Abstract

To support the use of selected plant extracts in Ayurveda, naturopathy, the antioxidant potential of the aqueous extract of Vincarosea ( VR ), Gymnemasylvestre ( GS ), Tinosporacordifolia ( TC ) and Emblicaofficinalis ( EO ) and their mixture (PHF) of Indian origin was investigated for in vitro antioxidant activity by using in vitro models like superoxide, hydroxyl radical scavenging activity and lipid peroxide inhibition assay. The results were compared with standard (ascorbic acid), a known antioxidant. The various phytoconstituents identified in the above selected plants extracts were poly phenols, flavonoids, terpenoids, tannins, alkaloids. The terpenoids were reported to protect lipids, blood and body fluids against the attack of free radicals, some types of reactive oxygen, hydroxylic groups, peroxides and superoxide radicals. The presence of these phytoconstituents in selected plants might be responsible for antioxidant activity with that of known antioxidant ascorbic acid.

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References

Khopde SM, Priyadarshini KI, Mohan H, Gawandi VB, Satav JG, Yakmi JV, Bhanavalikes MM, Biyani MK, Mithal JP. Characterizing the antioxidant activity of amla(Phyllanthusemblica) extract. CurroScist. 2001; 185-190.

Naik GH, Priyadarshini KI, Satav JG, Banavalikar MM, Sohani DP, Biyani MK, Mohan H. Comparative antioxidant activity of individual herbal component used in ayurveda medicine. Phytochemistry 2003; 63: 97-104.

HaIliwell B, Gutteridge JMC, Free Radicals in Biology and Medicine. OS'ed.), Clarendon Press. Oxford, 1985; 279 - 313

Wilson RL. 1988. "Free Radicals and Tissue Damage, Mechanistic Evidence from radiation studies

McCord JM and Fridovich I. Superoxide dismutase: An enzymatic function for erythrocuprein. Journal of Biological Chemistry 1969; 244: 6049-55.

Elizabeth K, Rao MNA. Oxygen radical scavenging activity of curcumin. Int J Pharm 1990:58:237-240.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry 1979; 95: 351- 358.

Kusum Devi V and Rehman F. 2002. "Neutraceutical Antioxidants"-An overview. Indian J. Pharm. Educ. 36(1): 3-8.

Jose JK, Kuttan R. Antioxidant activity of Emblicaofficinalis. J ClinBiochemNutr 1995.19:63-70.

Kapoor LD. Handbook of Ayurvedic Medicinal plants Boca Ratar FL: CRC Press Inc.: 1990: 200-201.

Naik GH, Priyadarsini KI, Naik DB, Gangabhagirathi R, Mohan H. Studies on the extract of Terminaliachebulaas a potent antioxidant and a probable radioprotector. Phytomedicine 2004; 11: 530-538.

PongtipSithisam, RoongtawnSupabphol, WandeeGritsanapan. Antioxidant activity of siameseneem tree (VPI209). J EthnopharmacoI 2005; 99: 109-112.

Ghosal S, Srivastava RS, Bhattacharya SK, Upadhyay SN, Jaiswal AK, Chattopadhyay U. Immunomodulatory and CNS effects of sitoindosides IX and X two new glycowithanolides from Withaniasomnifera. Phytother Res 1989; 2:201-206.

Bhattacharya SK, Satyan KS, Ghosal S. Antioxidant activity of glycowithanolides from Withaniasomnifera. Ind J ExperBioI 1997; 35:236-239.

Kawamori T, Tanaka T, Hirose Y, Ohniiishi M, Mori H. Inhibitory effects of d-limonene on the development of colonic aberrant crypt foci induced by azoxymethane in F344 rats. Carcinogenesis 1996: 17(2): 369-372.

Reddy BS, Wang CX, Samaha H. Chemoprevention of colon carcinogenesis by dietary perillyl alcohol. Cancer Res. 1997; 57:420-425.

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Published

2015-04-30

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Section

Research Articles

How to Cite

1.
M. GR, S. S, K. EK. In vitro antioxidant assay of selected aqueous plant extracts and their polyherbal formulation. Int J of Pharmc Res [Internet]. 2015 Apr. 30 [cited 2024 Oct. 18];5(4):86-90. Available from: https://ssjournals.co.in/index.php/ijpr/article/view/1927

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