A study of the in vitro free radical-scavenging property of Hedyotis diffusa using nitric oxide assay
DOI:
https://doi.org/10.7439/ijpr.v4i3.118Abstract
Introduction: Hedyotis diffusa, known as Snake Needle Grass, is a herb used in traditional Chinese medicine for the treatment of multiple ailments, especially cancer. It is found to possess anti-proliferative, anti-inflammatory and anti-ageing properties. Aim : To study the free radical scavenging property of the ethanolic extract of Hedyotis diffusa using Nitric oxide assay. Materials and Methods: A dried sample of Hedyotis diffusa was extracted using 85% ethanol. Various concentrations of the extract were mixed with Sodium nitroprusside(SNP) in Phosphate Buffer Saline(PBS). Then Griess reagent was added and the absorbance studied using a spectrophotometer at 546nm. Quercetin solution was used as the standard. Result: The herb exhibited maximal activity of 72.28% at the concentration of 1000 ?g/ml. The IC50 value of Quercetin and Herb was found to be 10.24 g/ml and 104.18 g/ml respectively. Conclusion: The ethanolic extract of Hedyotis diffusa demonstrated a concentration-dependent free radical scavenging property which can be postulated as the mechanism of action in its anti-cancer, anti-inflammatory and anti-ageing properties.Downloads
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Published
2014-09-30
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1.
Kagoo N, Darling C. A study of the in vitro free radical-scavenging property of Hedyotis diffusa using nitric oxide assay. Int J of Pharmc Res [Internet]. 2014 Sep. 30 [cited 2025 Mar. 14];4(3):138-41. Available from: https://ssjournals.co.in/index.php/ijpr/article/view/1269