Pharmacological investigation of Polyherbal formulation on Carbon tetrachloride (CCl4)-induced liver damage in wistar rats

Abstract

Objective: To investigate effect of Polyherbal formulation on Carbon tetrachloride (CCl ₄ )-induced liver damage in wistar rats. Methods: Wistar albino rats weighing 180-230 g either sex were used. The selected animals were divided in to four groups where each group consisted of six animals. Experimentally liver damage was produced by intra-peritoneal administration of CCl ₄ and olive oil mixture (1:1 v/v) (1 mL/kg, once daily, i.p.) for 7 days. Test Drug, Polyherbal formulation was administered orally for 7 consecutive days at 3 mL/kg, once daily. On 8 ^th day, Blood samples were collected to evaluate different serum biochemical parameters like Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), Alkaline phosphatase (ALP), Total bilirubin and Total protein. Liver from animals of each group was dissected out for histopathological examination. Statistical analysis: Statistical calculation were done by analysis of variance (ANOVA) followed by post hoc Dunnetts test, with significant level of p 0.05. Results and dDiscussion: Polyherbal formulation showed significant effect on activity levels of serum AST, ALT, ALP and total bilirubin level while comparing test group to disease control group. It also showed significant elevation in decreased level of serum total protein. Pre-treatment of Polyherbal formulation restored the hepatic architecture and protected the liver tissue from fatty degenerative changes by preventing the toxic chemical reaction induced by CCl ₄ . Conclusion: Finding of this study concludes that Polyherbal formulation (Vasuliv Syrup) has promising hepatoprotective activity against CCl ₄ -induced liver damage. It can be employed as safe and effective treatment for hepato-toxicity or liver damage.