Curcumin inhibits the proliferation of a human colorectal cancer cell line Caco-2 partially by both apoptosis and G2/M cell cycle arrest

Authors

  • Yohko Fujimoto Osaka University of Pharmaceutical Sciences, Laboratory of Physiological Chemistry
  • Satoru Sakuma Laboratory of Physiological Chemistry, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094
  • Chisato Maruyama Laboratory of Physiological Chemistry, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094
  • Tetsuya Kohda Laboratory of Physiological Chemistry, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094

DOI:

https://doi.org/10.7439/ijpr.v4i2.91

Abstract

The aim of this study was to assess the possible roles of the phytochemical compounds, curcumin, quercetin and resveratrol in the proliferation of human colorectal cancer cell line Caco-2. All three phytochemical compounds inhibited Caco-2 cell proliferation, with curcumin being more effective than quercetin and resveratrol. Investigations concerning DNA fragmentation in the nucleus, Bax and Bcl-2 mRNA expression levels, and caspase-3/7 activity indicated that curcumin induced apoptosis in Caco-2 cells through an increase in the Bax/Bcl-2 ratio and activation of caspase-3/7. Furthermore, the analysis of flow-cytometry showed that curcumin caused an arrest of G2/M phase in Caco-2 cells. These results suggest that curcumin suppresses Caco-2 proliferation partially via activation of the mitochondrial apoptotic pathway and cell cycle retardation.

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Published

2014-06-28

Issue

Vol. 4 No. 2 (2014): Apr-Jun

Section

Research Articles

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How to Cite

1.
Fujimoto Y, Sakuma S, Maruyama C, Kohda T. Curcumin inhibits the proliferation of a human colorectal cancer cell line Caco-2 partially by both apoptosis and G2/M cell cycle arrest. Int J of Pharmc Res [Internet]. 2014 Jun. 28 [cited 2024 Oct. 18];4(2):84-90. Available from: https://ssjournals.co.in/index.php/ijpr/article/view/1258