In-vitro antihelmintic effects of two Kenyan plant extracts against Heamonchus contortus adult worms

Abstract

This study was on evidence based information that Entada leptostachya Harms and Rapanea rhododendroides (Gil) Mez were used by the herbalists in Mbeere County, Kenya, for the treatment of gastrointestinal worms. The plants aqueous and solvent extracts were tested for their in-vitro antihelmintic activity against Haemonchus contortus adult worms. Of the eight plant extracts investigated, four extracts exhibited adult worm mortality greater than 50% while the other four afforded mortality ranging between 60-77%. E. leptostachya methanol extract was the most active (77%). Albendazole was used as a positive control drug while Goodwin s physiological solution was used as negative control. Methanol extracts for both plants exhibited the highest anthelmintic activity at the test concentrations of 25mg/ml. Although R. rhododendroides was ranked third in general usage by the herbalists, E. leptostachya was solely used for the treatment of intestinal worms. The present results demonstrated that E. leptostachya and R. rhododendroides plant extracts had antihelmintic agents, and justified their traditional use as alternative drugs for the treatment of heamonchosis in ruminants.