A comparative study of isolation, identification and grouping of streptococcal isolates from throat swabs of school going children applying different methods of extraction for antigen
DOI:
https://doi.org/10.7439/ijpc.v7i7.4249Abstract
Objective: The objective of this research was to collect the throat swabs from symptomatic and asymptomatic children and microbiologically positive sample were further taken up for grouping. Methods: Extraction of streptococcal group antigen by various methods namely enzyme extraction, fullers extraction, Lancefield extraction. Comparing the antigen extraction methods in identifying the sero groups of BHS.Comparative studies between conventional and enzymatic methods of extraction. Which one is ideal method of extraction and which one is common among the children. Results: Throat swabs were collected in the age group of 5-12 both symptomatic and asymptomatic children. Symptomatic such as rashes, throat pain, fever, cold, cough etc. Totally 361 throat swabs were collected of which 19 were BHS.18 isolates were typeable and 1 was non typeable. In enzyme extraction is not effective in the extraction of group D streptococci may be extracted with mutanolysin only after 4H incubation although effective for the extraction of some strains of group D streptococci.in fullers extraction it was seen to give an important clue to the structure of cell wall of gram positive bacteria in lance field extraction was easily performed specific and sensitive method. Conclusion: Group A streptococcal isolates were just 2 in no which shows that group A is not the predominant strain in our study. There by denoting the prevalence of rheumatic fever and RHD are becoming less. There was no cross reaction among groups were lancefield and fullers method were applied.in enzymatic method there was slight cross reaction noted between A and D in one isolate,F and G in one isolate and C and G in another isolate.Downloads
References
. Ryan K J ;Ray CG., Sherris Medical Microbiology (4th edition)MC Graw Hill
. Facklam R. What Happened To The streptococci overview of what happened to the streptococci: overview of taxonomic and nomenclature changes". Clin. Microbiol. Rev. 2002; 15 (4): 613
. Patterson MJ. Streptococcus. In: Baron's Medical Microbiology (Baron S et al, eds.) (4th ed.). Univ of Texas Medical Branch. ISBN 1996; 0-9631172-1-1.
. Facklam R. "What happened to the streptococci: overview of taxonomic and nomenclature changes". Clin Microbiol Rev 2002; 15 (4): 613
. Schrag S, Gorwitz R, Fultz-Butts K, Schuchat A. "Prevention of perinatal group B streptococcal disease. Revised guidelines from CDC". MMWR Recomm Rep 2002; 51 (RR-11): 1
. Huang PY, Lee MH, Yang CC, Leu HS. Group B streptococcal bacteremia in non-pregnant adults. J Microbiol Immunol Infect. Jun 2006; 39(3):237-41.
. Sendi P, Johansson L, Norrby-Teglund A. Invasive group B Streptococcal disease in non-pregnant adults : a review with emphasis on skin and soft-tissue infections. Infection. Mar 2008; 36(2):100-11.
. Phares CR, Lynfield R, Farley MM, Mohle-Boetani J, Harrison LH, Petit S, et al. Epidemiology of invasive group B streptococcal disease in the United States, 1999-2005. JAMA. May 7 2008;
(17):2056-65.
. Gardam MA, Low DE, Saginur R. Group B streptococcal necrotizing fasciitis and streptococcal toxic shock-like syndrome in adults. Arch Intern Med. 1998; 158:1704-8.
. Wu HM, Janapatla RP, Ho YR, Hung KH, Wu CW, Yan JJ, et al. Emergence of fluoroquinolone resistance in group B streptococcal isolates in Taiwan. Antimicrob Agents Chemother. May 2008;
(5):1888-90.
. Bayer AS, Chow AW, Anthony BF. Serious infections in adults due to group B streptococci. Clinical and serotypic characterization. Am J Med. 1976; 61:498-503.
. Colford JM Jr, Mohle-Boetani J, Vosti KL. Group B streptococcal bacteremia in adults. Five years' experience and a review of the literature. Medicine (Baltimore). Jul 1995; 74(4):176-90.
. Dworzack DL, Hodges GR, Barnes WG. Group B streptococcal infections in adult males. Am J Med Sci. 1979; 277:67-73.
. Farley MM, Harvey RC, Stull T. A population-based assessment of invasive disease due to group B Streptococcus in nonpregnant adults. N Engl J Med. 1993; 328:1807-11.
. Gallagher PG, Watanakunakorn C. Group B streptococcal bacteremia in a community teaching hospital. Am J Med. May 1985; 78(5):795-800.
. Harrison LH, Ali A, Dwyer DM. Relapsing invasive group B
streptococcal infection in adults. Ann Intern Med. 1995; 123:421-7.
. Jackson LA, Hilsdon R, Farley MM. Risk factors for group B streptococcal disease in adults. Ann Intern Med. 1995; 123:415-20.
. Lerner PI. Meningitis caused by Streptococcus in adults. J Infect Dis. 1975; 131 Suppl: S9-16.
. Lerner PI, Gopalakrishna KV, Wolinsky E. Group B streptococcus (S. agalactiae) bacteremia in adults: analysis of 32 cases and review of the literature. Medicine (Baltimore). 1977; 56:457-73. .
. Opal SM, Cross A, Palmer M. Group B streptococcal sepsis in adults and infants. Contrasts and comparisons. Arch Intern Med. 1988; 148:641-5.
. Persson E, Berg S, Bergseng H, Bergh K, Vals
. Schuchat A. Group B streptococcus. Lancet. Jan 2 1999; 353(9146):51-6. .
. Schwartz B, Schuchat A, Oxtoby MJ. Invasive group B streptococcal disease in adults. A population-based study in metropolitan Atlanta. JAMA. 1991; 266:1112-4.
. Trivalle C, Martin E, Martel P. Group B streptococcal bacteraemia in the elderly. J Med Microbiol. 1998; 47:649-52.
. Hickman M.E., Rench M.A., Ferrieri P., Baker C.J. Changing epidemiology of group B streptococcal colonization. Pediatrics 1999; 104:203-9.
. Moyo S.R., Mudzori J., Tswana A.S., Maeland J.A. Prevalence, capsular type distribution, Anthropometric and obstetric factors of group B Streptococcus (Streptococcus agalactiae) colonization in pregnancy Cent Afr J Med 2000; 46: 115-20.
. Rocha M.L.F., Beltrame L.M.R., Guedes S.C. Preval
. Sm
. Mocelin C.O., Carvalho D.A.F., Brites C., et al. Isolamento de Streptococcus agalactiae de gestantes na regi
. Miura E., Martin M.C. Group B streptococcal neonatal infections in Rio Grande do Sul, Brazil. Rev Inst Med Trop S
. Vaciloto E., Richtmann R., Costa H.P.F., et al. A survey of the incidence of neonatal sepsis by group B streptococcus during a decade in a Brazilian maternity hospital. Braz J Infect Dis 2002; 6:55-62.
. Beraldo C., Brito A.S.J, Saridakis H.O., Matsuo T. Preval
. Baker C.J., Edwards M.S. B streptococcal infections. In: Remington J., Klein J.O. (eds.). Infectious diseases of the fetus and newborn infant. 4th ed. Philadelphia: WB Saunders, 980-1054.
. Torres M.O., S
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