Antimicrobial Analysis and Structural Elucidation Of Active Compounds Of Dialium Indum Leaves Extract (Valvet Tarmarind)

Abstract

Antimicrobial analysis and structural elucidation were carried out on the purified leaves of Dialium indum. The Harbone method was used for the extraction. The extracts were separated using a combination of column chromatography and thin layer chromatography, which gave rise to the isolation of two fractions, these fractions were further purified using recrystallization. The melting point of each pure fraction was determined. The purified extracts were subjected to structural elucidation using various spectroscopic techniques which includes; FTIR, UV, H1 NMR, C13 NMR, DEPT 1350, COSY, TOCSY, HMBC and HSQC . The spectral analysis suggested the presence of Stigmasterol, and Lauric acid. The antimicrobial analysis (anti fungal and anti bacterial analysis) using the punched agar diffusion method was carried out on the isolated fractions comparatively with a standard drug Funbact-A cream (a broad spectrum antibiotic). A total of thirteen test organisms were used for this analysis amongst which were ten bacteria test organism and three fungi test organisms. The results from the average diameter zones of inhibition, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and fungicidal concentrations (MFC) showed that all the fractions were all active on the entire test organism with zones of inhibition ranging from 14mm-36mm comparatively. None of these fractions showed similar antimicrobial effect as the standard drug Funbact-A cream but individually could serve as anti microbial to diseases caused by these test organisms from their MIC, MBC and MFC.