In vitro antioxidant and spectrophotometric studies of a colonial ascidian Didemnum psammathodes

Authors

  • Priya D. Shanmuga Department of Chemistry, A.P.C. Mahalaxmi College for Women, Tuticorin 628 002, Tamilnadu
  • S. Sankaravadivu Department of Chemistry, A.P.C. Mahalaxmi college for Women, Thoothukudi
  • H. Kohila Subathra Christy Department of Chemistry, A.P.C. Mahalaxmi college for Women, Thoothukudi

DOI:

https://doi.org/10.7439/ijpc.v6i8.3466

Abstract

Didemnum psammathodes commonly available in Tuticorin coast was screened for its chemical value. Analysis of phenols and flavonoids were done by Spectrophotometry method. A maximum of 86.13% phenol and 69.07% Flavonoids were observed in Didemnum Psammathodes . This study is designed to examine the invitro antioxidant activity of phenolic compounds in the ethanolic extract of Didemnum psammathodes by DPPH method. In DPPH system the strongest radical scavenging activity was exhibited by the ethanolic extract (IC50 - 49.22) when compared to standard drug ascorbic acid (25.98).An increase in dose have significantly increased the percentage of antioxidant activity. This result free radical induced oxidative reveal that Didemnum psammathodes ethanolic extract a promising antioxidant potential against free radical induced oxidative damage. The present observation suggests need for further investigation of Didemnum Psammathodes so as to isolate secondary metabolites.

Downloads

Download data is not yet available.

References

Millar RH, Ciona LMBC. Mem, typ. Br. mar. Pl. Anim., 1953; 35: 1-123.

Terao J, Piscula M, Yao MC. Protective effect of epicatechin, epicatechin gallate and quercetin on lipid peroxidation in phoshpolipid bilayers. Archives of Biochemistry and Biophysics. 1994; 308:278-284.

Sravani, T, Paarakh, PM. Antioxidant activity of Hedychium spicatum Buch. Ham Rhizomes. Indian journal of natural products & resources. 2012; 3(3), 354- 358.

Kirtikar KR, Basu, BD. Indian medicinal plants, International book distributors, Dehradun, 2006, 993-994.

Meenakshi VK, Ph.D thesis, Manonmaniam Sundaranar University, Tirunelveli, 1997.

Khatiwora, E, Adsul BV, Kulkami, MM, Deshpande, NR, Kashalkar, RV. Spectroscopic determination of total phenols and flavonoids contents of Ipomoea carnea. International Journal of Chem Tech Research. 2010; 2(3):1698-1701.

Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoids content in propolis by two complementary colorimetric methods. Journal of Food Drug Analysis. 2002; 10: 178-182.

Brand-williams W, Cuvelier ME, Berset C. Use of free radical method to evaluate antioxidant activity .Lebensmittel Wissenschaft and Technologies.1995;28(1): 25-30.

Ebrahimzadeh MA, Hosseinimehr SJ, Hamidinia A, Jafari M. Antioxidant and free radical scavenging activity of Feijoa sallowiana fruits peel and leaves.Pharmacology online.2008;1:7-14.

Meenakshi, VK, Gomathy, S, Senthamarai, S, Paripooranaselvi, M, Chamundeswari, KP. Analysis of vitamins by HPLC and phenols, flavonoids by HPTLC Microcosmus exasperatus. European Journal of Zoological Research. 2012; 1($):105-110.

Cushnie TPT, Lamb AJ. Recent advances in understanding the antibacterial properties of flavonoids. International Journal of Antimicrobial Agents. 2011; 38(2): 99-107.

De Sousa RR, Queiroz KC, Souza AC, Gurgueira SA, Augusto AC, Miranda, MA, Peppelenbosch MP, Ferreira CV, Aoyama CH, Phosphoproteins levels, MAPK activities and NF kappa B expression are affected by fisetin. Journal of Enzyme Inhibiting Medical Chemistry. 2007; 22(4):439-444.

Gryglewski KJ, Korbut, J. On the mechanism of antithrombotic action of flavonoids. Biochemical Pharmacology. 1987; 36:317-321.

Schuier M, Sies H, Illek B, Fischer, H. Cocoa-related flavonoids inhibit CFTR-mediated chloride transport across T84 human colon epithelia. Journal of Nutrition. 2005; 135(10): 2320

Spencer P, Jeremy, E. Flavonoids: modulators of brain function? British Journal of Nutrition. 99: ES60

Downloads

Published

2016-08-30

Issue

Section

Research Articles

How to Cite

1.
In vitro antioxidant and spectrophotometric studies of a colonial ascidian Didemnum psammathodes. Int J of Pharm Chem [Internet]. 2016 Aug. 30 [cited 2026 Jan. 4];6(8):192-5. Available from: https://ssjournals.co.in/index.php/ijpc/article/view/3466